FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063087
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20390.1917999185391224No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA16460.15483210687366133No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC16090.15135167676775277No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA14940.14053412373587487No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC14230.13385546055967198No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT13650.12839965120446398No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG12580.1183346236008906No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT12460.1172058354584338No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG12250.11523045620913433No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT11720.1102449752466167No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA11460.1077992676046269No Hit
ATTATATTCAATATGGAAAGAATAAAAGAGCTAAGGAATCTGATGTCGCA11400.1072348735333985No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC10960.10309598367772346No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA10820.10177906417819049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA950.0122.12151
GTAGAAA8450.055.776651
CAAGGTA3650.049.6547979
ATATTCA4000.048.9394044
GTACTTA604.941979E-448.3397671
TTATATT4100.045.97742
CAAGGGT5350.040.6519628
TTAGTAG1354.966605E-737.5975951
TACTGAT2350.037.0192347
TATACTG2401.8189894E-1236.251415
AGGGTGT3750.034.8013536
TCGTACT3800.034.343442
ACAAGGG6550.034.3110857
CGTACTC4050.034.013673
ATATAGT5550.033.9684831
GTACTGA2800.033.6620256
TAATCAC2603.6379788E-1233.459698
AGTGGTA5700.033.071465
TAGATAT2850.033.071463
GTGGTAT5700.033.071466
Produced by FastQC (version 0.11.5)