FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAGAAAAGTGCATATAGCTAAAGTTGATTAATCAAATTTTCAAAAT12.7027027027027026No Hit
TTCTACCACAAGTGTAACAATGAATGCATGGAAAGTGTGAAAAATGGAAC12.7027027027027026No Hit
CAAGTGGTGAGGGTATAATTTACAATTCATATCTTTCATATATAGTTTTG12.7027027027027026No Hit
CAAAGATCTACAAAACTTATTTTGAAAAAGTCGAAAGGTTAAAACATGGA12.7027027027027026No Hit
GTACTGAATTGTTAAGCAACTGTATTCTCATCAGTGGCAAGATCACATCT12.7027027027027026No Hit
AGCAGGCAGAGGGAGAAGGGGAAGTAGCCTCCCTACGGCGCAAAGCGCCC12.7027027027027026No Hit
TCCATCGACAAAGTGATGGGAATGATCGGAATACTGCCCGACATGACCCC12.7027027027027026No Hit
ATCAAAACACCGTGGTGAACTAGTTCGAGTAGGACCTGCTTGCTGGGGGT12.7027027027027026No Hit
GTGTGGGTGTGTGAGCGAGTGTGAGCAAGCATAAGCATGTGTGTAAGGGC12.7027027027027026No Hit
GGATTTGAACTCATGACCCAAGATGAAGACCTGAGTTGAGATCGAGTTGA12.7027027027027026No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12.7027027027027026No Hit
CTTAAAAGTCATAGGCCACAAACTGGGCCTGGAGATACTTCACACCCCCA12.7027027027027026No Hit
CAGCTGCACAAAGAGCAATGATGGATCAAGTGAGAGAAAGCCGAAACCCA12.7027027027027026No Hit
ATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCGGGAGAC12.7027027027027026No Hit
GTGGGAGCCAGAATACTAACATCGGAATCGCAATTAACGATAACCAAAGA12.7027027027027026No Hit
ATGGATGAATGTGACGCGAAGTGTCAAACACCCCAGGGAGCTATAAACAG12.7027027027027026No Hit
CATAAAAGGAAGATCTCACTTAAGGAATGACACCGACCTGGTAAACATTG12.7027027027027026No Hit
AATTACAGCTTTTCAGATCTGCTAGTGATTTAGTCACTTCAAATAAGGTG12.7027027027027026No Hit
GACAGAAACTTCTGGAGGGGTGAGAATGGAAGAAAAACAAGGATTGCTTA12.7027027027027026No Hit
GAAGAGTTAACAATAGTAGGAAAAAGGGCAACAGCTATCCTCAGAAAAGC12.7027027027027026No Hit
CCTATCTTCTTTGACCAGAATGAGGAATCCCCTTAGAAAAGCGGATTCCA12.7027027027027026No Hit
GAATAAAAGAGCTAAGGAATCTGATGTCGCAGTATCGCACTCGCGAGATA12.7027027027027026No Hit
GTAAAGTGCTGGGGTCTTTAGGAAGAACACAGTGAATAAATACCCAAAGC12.7027027027027026No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTAAGTATCGAGA12.7027027027027026No Hit
GGATGCTGGCCTCCGGGCCAGGGAAGGAGAGTGAGGCCAGCCCAGTGGGA12.7027027027027026No Hit
CTCTGTTAGCGCTTGTGGTAAAGGCAGCTATGTGAGACTCGGCCTTTGAT12.7027027027027026No Hit
CCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGGTGGGTTGCTTATA12.7027027027027026No Hit
GCCCGATCCTTGATAATTCTGATGATGATAACCAGAACATCACACGATCA12.7027027027027026No Hit
GCTGCCATATGCACACATTTGGAAGTGGGGTTCAGGTATTAGGAGTTCCA12.7027027027027026No Hit
CCATGGAAAAAGGTTTGCGGACAGAGAGCATACGCAGGCACAGACTTGCG12.7027027027027026No Hit
GAATGGATATCAAATACTACCATTGACACAACTACTAAAGTCCAAGTTTG12.7027027027027026No Hit
GAGAATATGATCCCAGCATGAGAATCCAGCTTCTACGGGAATGACTGGCT12.7027027027027026No Hit
CTCGAGGAAGAAAGCAGGGCTAGGAGGAAAACCAGACTATTCACCATAAG12.7027027027027026No Hit
GTTCGCTGTTGCAGGTGGGACTTCCAGCAGTGGGCGATCCGCTAGCACTT12.7027027027027026No Hit
GTGAGAAGAGTATACTTCCGGTTTCCCTTGTGGGTGCATAGGGCCATATG12.7027027027027026No Hit
CAACAGATGATAAATCTTATGTACCATGGAGTATGTTTCTGCTCGCTTAA12.7027027027027026No Hit
GTCCGAGAGAGGCGAAGAAACAATTGAAGAAAGATTTGATATCACAGGAA12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)