FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTAATTACC13.8461538461538463No Hit
TGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTC13.8461538461538463No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA13.8461538461538463No Hit
CTACAAACACAGACTTAATTTATTATTGTGTTTAGTTAGAGAATGAAAAG13.8461538461538463No Hit
GTGTGAGGCGAACGTGATAACCACTACAGTAGACCCAATTTCTGTGAATG13.8461538461538463No Hit
GTGTGTGGTGTGTGTGGTGAGGGTTAGGGTTGTCTAGCTGCCCAGTGCCC13.8461538461538463No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC13.8461538461538463No Hit
CATGCTTACTGCTCACTTCAAAATTCACTTCAGTAAGGATGCTGAGAATG13.8461538461538463No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT13.8461538461538463No Hit
ATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAAT13.8461538461538463No Hit
TGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGG13.8461538461538463No Hit
GTCTATACGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA13.8461538461538463No Hit
TTTTTAAACAATTCGACACTAATTGGTGGCCATCCGAATTCTTTTGGTCG13.8461538461538463No Hit
GGGAAAGGGAAGTGACACACTCACCTTCATAGGCGTGAGACTAGATGAAG13.8461538461538463No Hit
GTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTCTTTGAAAACAAAT13.8461538461538463No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT13.8461538461538463No Hit
TTATAGCCCCTGGTGCCTACCCCAGCTTCTCCAAGGGTGGGGACAGCCAG13.8461538461538463No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG13.8461538461538463No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATAAGATCCGAAA13.8461538461538463No Hit
AATGGGAGCTGCAGATAAGTGAGAAACGACAAGGGCTCTGGCAGGCGCAG13.8461538461538463No Hit
CATCCGGTCAGCTGATTTGTGAACGACGGAGCAGCTCCCTTTCTAGCGGG13.8461538461538463No Hit
AAGGCTGCTAGCTTGAGAAGGGTAACCACTATCCACACACCACACCCCCA13.8461538461538463No Hit
GTATTCAACAGTCTATATGAGTCTCCACAACTTGAGGGGTTTTCGGCTGA13.8461538461538463No Hit
GGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCACACAT13.8461538461538463No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG13.8461538461538463No Hit
GGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)