FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAACACGTTCCATTGTCTGAACTAGGAGTATCCACAATGTACGACCA16.25No Hit
AAACAGTTCCTGCTGCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCC16.25No Hit
GTATAGCCCTGGGAAATTTAAAAATTCCGTTGACTCATGTCGTGACAATA16.25No Hit
GATAAACCAAACATTTCACATCACAAGGAGAGTAAGAGCCCCAATGAACA16.25No Hit
GATTCCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCCAGCATTAAGC16.25No Hit
GAATAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCT16.25No Hit
TGCAGGTACTGATCCAAAATGGAAGACTTTGTTCGACAATGGTTCAATCC16.25No Hit
TTCTGTGCCTGTTGACTATGATGTCTACTTGATTTAAGTGATTTGCCTGA16.25No Hit
GGCATGAGGACCTCCTTCCCCAGCAAGGTCGCAGCTTGAACATCAGGAAC16.25No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA16.25No Hit
GCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG16.25No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG16.25No Hit
GTAGTGGACAAACCCCCGTAGCTTATACCACAACGTTGCGTCCAGATGGG16.25No Hit
GGCAGTAGGCAGGGGTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGTAG16.25No Hit
GTGGTGGGCTGTACAGGGCCTATAGTATTGGGGGTAGGAGCTACAGTAGC16.25No Hit
AGCAGGTACTGATCCACAATGGCAGACTTTGTGCGACAGTGCGTCAATCC16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)