FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT60.38860103626943004No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT30.19430051813471502No Hit
ATCTTGGTGTCCTTGGTTTTCATTAACTTGATACAGTAATTTTAAAAAAA30.19430051813471502No Hit
GTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATTTGATTCCCT30.19430051813471502No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC20.1295336787564767No Hit
GGCCTACCTCCCACAGCCCTGGGACGCCTATGGGGCCAGGCCAGCACTGG20.1295336787564767No Hit
CCAAGCAGGGAGCCCGATGCAGGGCTCGATCCCAGGACCCTGGAATCATG20.1295336787564767No Hit
TCCCTACAGAGTTAGATGGAAAAGTGTGCGGGAGGAATTCAAAGAAGGCA20.1295336787564767No Hit
ATTTTAAAACATTACCTTAGCAGTCATTTACCCTCAAGTTCTTTTTTATG20.1295336787564767No Hit
GCTCTGAGCCTTTTATGTAACAACAGTCAGTGTGCAAATCAGTGTTCTGG20.1295336787564767No Hit
ACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTT20.1295336787564767No Hit
ATAGTAGGATCAAGAACAACTAGTAAGGGAAGATAACTATCAGGGGATTA20.1295336787564767No Hit
CCCTAAACCTCTCTGGGGATCTGACGTGTCTCCCTTCCCAATATCACCCC20.1295336787564767No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC20.1295336787564767No Hit
GTTCACACATGGATGCATGTGGGTAGTAACAATCAGGGCATTCCTACTTC20.1295336787564767No Hit
ATCATGACCTGAGCCAAAGACAGAAGCTTAATGACTGAGTCATGAAGGCA20.1295336787564767No Hit
ATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACA20.1295336787564767No Hit
GTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGAC20.1295336787564767No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG20.1295336787564767No Hit
CCCCATGCCAGGCTCCACGTTTGGCATGGTTGAGGGGAGCAGGGACTGCT20.1295336787564767No Hit
ATGTAACATCGTAACTTGAGGTTCAAAAACCGTGGCAGAATGAACGGTCT20.1295336787564767No Hit
CATTTTATTCTTCCTCTTCCTCCAAGTATGTTTACTTTCCTGCCCAAGAC20.1295336787564767No Hit
AGAGAAAATAGAATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTG20.1295336787564767No Hit
GTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGG20.1295336787564767No Hit
AGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGGGGGCAGTGGACGGGG20.1295336787564767No Hit
CCCCTAAATGGTCCATTTTAAAGATAAAATTTGATCATTCCCAAAACCTA20.1295336787564767No Hit
GTATAAAAATAGAATAAGATTGAAGGGTGCTTTTATTTTTACTTCTTAAA20.1295336787564767No Hit
GATATAGAGATCTTAGAAGCGATAAGAGAAAAGGAGTTAAGATTATTCAC20.1295336787564767No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20.1295336787564767No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA20.1295336787564767No Hit
GAAAAGAAGAGTGGCTATTGCACGGACATGCTTGGGCAAGACCGGAGGAA20.1295336787564767No Hit
GGAATACAGAGTGGAGGACATAAGAGGAAACACAGGGAGGGCCTAGGCGA20.1295336787564767No Hit
AAATAATGCAGTTGTTCATTTGATCTTTTTTGAGGTCATGGACCTCTTCA20.1295336787564767No Hit
GTTTGTGTGCCGTGTGCACTGGGAAAGAAAGTCCGTTTTCTTGTTGGTAT20.1295336787564767No Hit
GCCAAGCTGCACCATTCTGCATTCCCGCGAGCACATAACAAGAGCACCCG20.1295336787564767No Hit
GGGTACAGAGGCATTCCGTTCTCACCCCGACCCTTGAGCCTCTCCCCTTC20.1295336787564767No Hit
TCAGGAGACAATGCAAAAATGAAAGTTAGGAATTCAACGAGGAAGGGATG20.1295336787564767No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)