FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822598
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12610.1532948049958789No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11160.1356677259122925No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10480.12740123365240372No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC8620.10478994600035497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.064.805681
CAATTCG4300.052.266439
GGTCGTT2900.044.999137
GCGAAAG4050.041.1845632
CGAAAGC4100.040.6823123
ACAATTC6500.039.037718
AGGTCGT3700.035.2803156
GACAGTA3950.034.8727467
AGTAGAA7350.034.533641
GTAGAAA7600.033.3976632
TATATTC5350.032.5326273
GGATAGC3850.032.0221025
TCGTTTT4000.030.8119079
TAGAAAC9850.030.1862583
TTTAAAC9050.029.649323
GTACTGA4350.028.3414026
CTATTAA2903.947207E-1027.5078281
GTCTAAG4000.027.1952421
GTATTAA2756.7411747E-926.3711431
AACAATT10050.025.9696467
Produced by FastQC (version 0.11.5)