FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG70.31919744642042863No Hit
ATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAG60.27359781121751026No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60.27359781121751026No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT50.22799817601459188No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA50.22799817601459188No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.1823985408116735No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG40.1823985408116735No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.1823985408116735No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG40.1823985408116735No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.13679890560875513No Hit
CTCTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTA30.13679890560875513No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.13679890560875513No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.13679890560875513No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT30.13679890560875513No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT30.13679890560875513No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.13679890560875513No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG30.13679890560875513No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.13679890560875513No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.13679890560875513No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT30.13679890560875513No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC30.13679890560875513No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT30.13679890560875513No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.13679890560875513No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.13679890560875513No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)