FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT14.3478260869565215No Hit
GGAACAGTCGAGCTGCGTCTCCCCAACTTGAGGGGGTTGCGTCTGAATCT14.3478260869565215No Hit
TGGCAATAAGAATTCTTTATGTCTAGAACAGCCATGGCGGAAGTTTCATG14.3478260869565215No Hit
GTCCCGCACTAGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA14.3478260869565215No Hit
GACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCCTCCA14.3478260869565215No Hit
GAAGCTGTGGGATCAACCCCAATCAAAGGTAGGGCTCTTAGTATCAGAGG14.3478260869565215No Hit
GCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAG14.3478260869565215No Hit
TCACCAAGGTGTTCTGCAGGGTGTATTGTATGTTTTATCAGAATGGTTAG14.3478260869565215No Hit
AAAAAAGCCCGTGTAAAAAGAATTCCACAGCAATTTTTCTCGATAAGCAT14.3478260869565215No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14.3478260869565215No Hit
ATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAAC14.3478260869565215No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTCCATGTGTG14.3478260869565215No Hit
TCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTA14.3478260869565215No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA14.3478260869565215No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCAGGGCCCTAGACACCATG14.3478260869565215No Hit
GCCCTGCATCAGGCTCCCTGCTCAGCTTCTCCCCAGTCCCCCGACCTGCC14.3478260869565215No Hit
TTTTTATACAAATGCCCACTAATAGAAGGCCCTGCGCTTTCTTTAGGAGG14.3478260869565215No Hit
CATGTAAGATCCTAAATCCTCCGGCAAGTGGACAGATGATTGCCAATATT14.3478260869565215No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT14.3478260869565215No Hit
GAGAGATCTAATTTAGCAGTCCCGCACTCTCGAGATAATCACTAAGACAA14.3478260869565215No Hit
TGACAAGAAAGCCATCGTTATTGAGGGGACTTATTTCCATGTGACAGTTA14.3478260869565215No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGACGAAAACCCCTCAAGTAAT14.3478260869565215No Hit
CTAAGGATGTCCCCCATCCTTACTCCTCCAATCTGTGTTCTGTGGCACAT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)