FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502287
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA5250.10452191675277282No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC5120.10193375500460891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.049.6149021
CCGCGTC450.009636546548.333103145
GCGAAAG2650.035.569412
CGAAAGC3251.8189894E-1229.0027473
TAGGACC1855.8043042E-627.4350324
CTATACT4300.026.97934
GATATAC1358.0679177E-426.857071
GTATAAT1409.976324E-425.897891
GTATTAG1701.0289318E-425.5932081
ATACTTA3709.094947E-1225.4753886
GTATAAG2001.0624894E-525.3799321
CCTTAAT2301.0994227E-625.2222921
TATATAC4900.025.1554433
TATAGAC6350.025.1204912
ACTATCC1450.001225627824.9998788
GGTTATA1751.2528549E-424.8619731
ATAGACC3551.546141E-1024.5093633
GTATTAC4500.024.1713641
TCCCTAT1801.5168147E-424.1713641
CCTATAT1500.001491550824.1713621
Produced by FastQC (version 0.11.5)