FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGCTCTCCTCCAAGCCTGGGTGCTTGGAGGAGGGGTCGACTCCAG11.2048192771084338No Hit
CTCGTACATTTTCTAATACAAAATAAAAAATTAACACAGGAATGGAGAAA11.2048192771084338No Hit
TCATTGATGACGAATAAGTTATTGTCGACATCTCAGATCCTTGCCGTTCA11.2048192771084338No Hit
ATTCAAGCTGGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATT11.2048192771084338No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.2048192771084338No Hit
CTGCACTACCACATCAAGTGCTCTAAGGCCTATATCCACACACGTATGCG11.2048192771084338No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA11.2048192771084338No Hit
TCCTCATTTCTCACTTCTCCTCCTGGAGTGTACATCTGCTCCCAGCAAGT11.2048192771084338No Hit
ATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGG11.2048192771084338No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT11.2048192771084338No Hit
GTGTGGAGTGCTGGGCGGGGTAGGAGCGGTGGCCACTTCCCTCAGGAGCT11.2048192771084338No Hit
GTAGTAAACGTATCCGAAGTTAAAAGTGAGGCTTTGTGTTTCCAAGATAG11.2048192771084338No Hit
CTCTTAATGTGTAAACTCTTACAGACCACACTTAATAAACTAACTCAATA11.2048192771084338No Hit
ACACATTGCAAGGTTTAAATAAATACTTATAAAGTTTGTTGAATAAATGA11.2048192771084338No Hit
AAAGAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAA11.2048192771084338No Hit
GGGCTTGATCTTATGCCGCTGAGATTATCACCTGAGCTGAAATTAAGAGT11.2048192771084338No Hit
GCCCGACATGCCCCCACGCACGGAGAGGTCGCGGAGAGGGATAAGCGTCA11.2048192771084338No Hit
ACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTT11.2048192771084338No Hit
CATACAGAGAGCTTATATCCTATCTTCTTGATCTCTGGATGGACACTTGG11.2048192771084338No Hit
CCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTGCAACTACACATT11.2048192771084338No Hit
GTCCTGGGATGTTGATGGCCTGGAACAACCTGCTAAGTAAAGTATTGGTA11.2048192771084338No Hit
TTATTAATACCAAACAAATGGGATGCTTGAAAGGCAGGAGACCCAGATTC11.2048192771084338No Hit
GGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTC11.2048192771084338No Hit
GATAGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAAGTGAGG11.2048192771084338No Hit
ACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATAGAAAG11.2048192771084338No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG11.2048192771084338No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA11.2048192771084338No Hit
GAATTGGAGAACACTGGGTTTTTTCAATTCCAAATGCAGTGTTTACATTA11.2048192771084338No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA11.2048192771084338No Hit
ATAATAAAGATTAACCATCATGTAAATAATTAAATTATAGGTAAAAACAA11.2048192771084338No Hit
GCAAGAGAGAGAGGGGCAGAGGCAGGGGGCCATAATGGCCCCCATGCTTC11.2048192771084338No Hit
CTGACCCCCAGTTGCCCACGAGGGCGCCTGGTAACAGCTTTCAGAGACTT11.2048192771084338No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG11.2048192771084338No Hit
GGGCCGCATTTCTTCAATTAACCACATTAGTTCCTAAAATTTATGTCCCA11.2048192771084338No Hit
GGGTGCCAAAAACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAG11.2048192771084338No Hit
ATGGAACGTTATTTCTCTTTTGAGCTTCTTGTATAGTTTAACTGCTCTAT11.2048192771084338No Hit
GATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGA11.2048192771084338No Hit
CTCCCAGCCTGCCTGACCTGGGCTGGATGTCCCCCCTAACCCTCTCTGGG11.2048192771084338No Hit
GATCGAGTCCCGCATCAGGCTCTCTGCTCAGCGGGGAGCCTGCTTCCTCC11.2048192771084338No Hit
GCGTTCACCCTGATCCTCCCTGAGCACCAAGGCCCTGATGGGGGAACCCT11.2048192771084338No Hit
ATGTCTCTTTGGGGGATCCGCTTTTCGGCCTCCTTCGCAGGCCTCTGTCC11.2048192771084338No Hit
CATATGGTCCTTCCATGGGGAAGATGGAGCCAGGAGAGTCGGAATTAGAG11.2048192771084338No Hit
GATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACA11.2048192771084338No Hit
GTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGC11.2048192771084338No Hit
ATTCTATTCTATTTTCCTGAAGGTGGTGCTCCAGTTCAAGTTTGTGGTTT11.2048192771084338No Hit
CCCTTGGGTGTTTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA11.2048192771084338No Hit
GGTGAAAATATGGCAGCATAAAAAGTAGACTTTGATGACTGCAAAGATGT11.2048192771084338No Hit
GTTAATATTCTCTGGAATACGAAATAAATTTATAAACTTGCATTTGCAAA11.2048192771084338No Hit
GAACCCGAGAAGAGCACAGGCTCCTGCCCCACCGAAGTCACCTGTCGATG11.2048192771084338No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT11.2048192771084338No Hit
TAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGA11.2048192771084338No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC11.2048192771084338No Hit
GCTCCTGCTGCTGCTCCTGCTGCTGCCACTGCTCCTGCTCCTGCTGCTGC11.2048192771084338No Hit
TAATCATAGTGGATCTGAAATTAGAATATCCGCACTTCCATTAGAGGAGA11.2048192771084338No Hit
GGACGGCAGGCGTATGTAGAAAATATTAATGTACCTCTTTTATAGATACA11.2048192771084338No Hit
CAGATATTCAATCTGGAGAGCATAAAAGAACTGAGAGCTCTAATGTCGCA11.2048192771084338No Hit
GCTGCTGTTGGATGTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGAC11.2048192771084338No Hit
GTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGC11.2048192771084338No Hit
CCTCCATACAGCCATCGAACAGGAACACGATACACAATGGACACAGGAAA11.2048192771084338No Hit
GACTCAGGTAGGAGCCACTGCTATGAAAATCACACCAGTATGAACCAGGA11.2048192771084338No Hit
GGCTCAGGGTGAGGGTGACAGCCTTTGGCAGGAGGGATCCCTGAGATTCC11.2048192771084338No Hit
TGCTGAGACCACGGGCATCTGCGAGGGGAAATGCTGTCTTTCTGAGAGGC11.2048192771084338No Hit
CCCTCGTGGTATCTAACGCCTGGACGCCGAGCAGCCGGAGCAGAGCTGGG11.2048192771084338No Hit
GGGCAGGACACCTATAGTCACTAGAGCAAGCCAACAAAATAAAATATGAG11.2048192771084338No Hit
GTGTCAAATGTCCCAAGCACATCCCGAATTTGCTGGAACAGTGTCCTTAC11.2048192771084338No Hit
GCCCTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAG11.2048192771084338No Hit
CATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCA11.2048192771084338No Hit
CTATTTCCTCTGATTAAAAAGAGAAGCAATTATCACTAGCCCCATTTTAC11.2048192771084338No Hit
ACAACACACAGAGATTTCTTTCACAATCACTGGGGCCCACACTAAGTGGA11.2048192771084338No Hit
CAGCCAGCTCCACTGCCACTGAATTCCAGCATACTCACCTCTGCCAGCTG11.2048192771084338No Hit
CCCCCCACATCTATTAAAAAAAAAAAAAAACTTAACCAAACTTCTAGGCT11.2048192771084338No Hit
GCGTTGGGGGACTATAGGCACGGCGCTGGCAATGGTCTTTTTTATTTATT11.2048192771084338No Hit
TCCCGGGACCCCGGGATCACACCCCAAACTGAAGATTTAACCAACTGAGC11.2048192771084338No Hit
TCCCTGAACCTATGCCTATGTTAGGTGAGTGGTGACCAGGAATCAAGGGT11.2048192771084338No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA11.2048192771084338No Hit
GTCAAACAACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAA11.2048192771084338No Hit
ATTCACACTCACGAGTACCCGTGCATTTTATGCAATTCCAAGTATCACCA11.2048192771084338No Hit
GATCTGTGTCAGGAATGATGATCCTTTCTGTAGTTGTAAAGCTAGGCAAG11.2048192771084338No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG11.2048192771084338No Hit
TGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGAT11.2048192771084338No Hit
GATCAAGTAATAGACATTATAAAAAAATGAAACGATGAGCTGTACTACAT11.2048192771084338No Hit
TTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCT11.2048192771084338No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTTC50.0145.09
GGCCGCA50.0145.02
CCGCATT50.0145.04
CATTTCT50.0145.07
GCCGCAT50.0145.03
AGACTAG50.0145.0145
CGCATTT50.0145.05
GCATTTC50.0145.06
ATTTCTT50.0145.08
GGGCCGC50.0145.01
Produced by FastQC (version 0.11.5)