FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCACCTTTGGCTTG15.555555555555555No Hit
ATAAAATATGGGCTATACTGTGACAATAGGAGTGGCTGTTTGGTTTGCTG15.555555555555555No Hit
TCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCAT15.555555555555555No Hit
TGCCAGCATGTCCGTCAGTGAAAACATACCCTGTAGCAGAGCTAAGAGCC15.555555555555555No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGA15.555555555555555No Hit
GGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATT15.555555555555555No Hit
GTATACGTGCTATTTACATAATAAGGAAAATGTACTTTAAATAGATGTAT15.555555555555555No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGCAT15.555555555555555No Hit
CAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATAT15.555555555555555No Hit
GGGCCAAGGTGGTGTCTCTAAGCTATTCACCTGGTGCACTTGCCAGTTGC15.555555555555555No Hit
GGGCCGAAGGTACAATGTTTCAACCTTTCGAACTTTTCGAAATAAGTTTT15.555555555555555No Hit
CGGTGAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCATTT15.555555555555555No Hit
TACCTAAGTGGCTCAGCAATAAAATTACTGTTTTTTTTGTGGCTTACCTT15.555555555555555No Hit
GAGTCAGGAAGGCTAATAGATTTCTTACAGCATGTAATGGATGCAATGCA15.555555555555555No Hit
CAAATGGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGA15.555555555555555No Hit
GCTTGTATTCCCTCATGGTTTGGTGCCTTCACTATGAGAGCAAAATCGTC15.555555555555555No Hit
AACCATTAGAGCAAATCCAGAAAATGAGTAAACCAACGTACCATAACATT15.555555555555555No Hit
CCCACATGATAAACAATGCCCTTGGACCTGCAACGGCCGAGATGGCGGTT15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)