FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_F03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_F03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614247
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8140.13251997974756083No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC7970.1297523634629066No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7280.1185190973663689No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA7080.11526307820795217No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT6750.10989064659656458No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT6690.10891384084903954No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT6660.10842543797527704No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6210.10109939486883941No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA6180.1006109919950769No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6150.1001225891213144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT651.3964382E-766.928034
AGTAGAA5000.050.7578961
GTAGAAA5700.047.0648882
CGAAAGC1109.945143E-846.1397823
AGCGAAA951.9176114E-645.7965931
CCTTATA3400.044.782732
TTATACT3950.038.5471654
CAATTCG2450.038.4691059
GTGGTAT3350.036.793776
TATATTC4350.036.6693883
CAAGACG2201.6370905E-1136.252686145
ATAGTGG3400.036.2526863
ATATAGT3600.034.2414361
CCCTTAT5000.031.9049631
ATATTCA5250.031.764264
TAGATAT1601.8744722E-631.7211023
TAGACCC2101.4368197E-831.0737324
TGTATAC1402.7435744E-531.0737322
GCGCACG950.004724178530.528576145
ATACGTG1204.0544805E-430.2105715
Produced by FastQC (version 0.11.5)