FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850525
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG450.00963277948.3407822
AGCGAAA2450.041.4398271
GTATATA12650.036.1165281
CATACAT8200.030.944438
GTATAAG3050.030.9100361
CGAAAGC3350.030.3031773
TACATAC8200.030.0656096
GCGAAAG3650.027.8125042
TATACAT10150.025.718254
GTATTAC8600.025.297571
CTAGTAC1751.254197E-424.8609733
TATATAT10250.024.0524862
TAGAAAC8450.024.027373
TCGTAAC3052.0341758E-823.7699629
TATACAG6500.023.4266875
TATAGAC8700.023.3369272
ATATATG6850.023.288263
ACATACA11050.022.963297
CCTTATA2852.7736496E-722.8982642
GTATAAT3203.3744072E-822.6624071
Produced by FastQC (version 0.11.5)