FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT60.3527336860670194No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC50.29394473838918284No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT50.29394473838918284No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT40.23515579071134626No Hit
GCTTAATGCATCCTGTGCAGCAATGGATGATTTCCAACTAATTCCCATGA40.23515579071134626No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC40.23515579071134626No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC40.23515579071134626No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG40.23515579071134626No Hit
ATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGCACCAAACGG40.23515579071134626No Hit
CATTAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATT40.23515579071134626No Hit
CAATAATATTTGAGGCAAATGGAAATCTAATAGCGCCATGGTATGCTTTC30.1763668430335097No Hit
CCACAATATCAAGTGCAAAATCCCAATGATACTTGCGGCAACAACAAGAG30.1763668430335097No Hit
GTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG30.1763668430335097No Hit
CTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAAAT30.1763668430335097No Hit
GTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACAGG30.1763668430335097No Hit
AATATGCGCAACCTGTGAACAGATTGCTGACTCCCAGCATAGATCTCATA30.1763668430335097No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA30.1763668430335097No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA30.1763668430335097No Hit
ATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATT30.1763668430335097No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA30.1763668430335097No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG30.1763668430335097No Hit
CTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA30.1763668430335097No Hit
GATAATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGCACCAA30.1763668430335097No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA30.1763668430335097No Hit
GAGCTAGGGTGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC20.11757789535567313No Hit
CCTCAAAGCCGAGATCGCACAGAGACTTGAAGATGTCTTTGCTGGGAAGA20.11757789535567313No Hit
CATTGAAGGGGGGTGGACTGGAATGATAGATGGATGGTATGGTTATCATC20.11757789535567313No Hit
CCATTGGGACTCACCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTT20.11757789535567313No Hit
GCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCAGCAGAGGCCATGGA20.11757789535567313No Hit
GATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAATGGTGTTG20.11757789535567313No Hit
GAATAGATTCATCGAGATTGGAGTGACAAGGAGGGAAGTTCACATATACT20.11757789535567313No Hit
GTCCTATAGGTGAAGCTCCGTCTCCCTACAATTCAAGGTTTGAATCAGTT20.11757789535567313No Hit
TCTCTGTAGAGTTTTAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC20.11757789535567313No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGC20.11757789535567313No Hit
GGGTACACCATGGATACAGTCAACAGGACACATCAGTACTCAGAAAGAGG20.11757789535567313No Hit
CACTTGGCTGGCTTGAAGCAGTTTGAAAGGGTCTATCCCTACCAAAGAGT20.11757789535567313No Hit
ACTATGTTCCTAGCAGCAATAATTAAGCTTTGATCAACATCATCATTCCT20.11757789535567313No Hit
CTTCTTAGAAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTT20.11757789535567313No Hit
GGGTGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGGCT20.11757789535567313No Hit
CAACAACCAATCCATTAATAAGACATGAGAACAGAATGGTTCTGGCCAGC20.11757789535567313No Hit
GTCCATATTATTTGGATCCCCATTCCCATTAAGGGCATTTTGGACAAAGC20.11757789535567313No Hit
GTCTAACATAGGGGACCAAAGGGCCATCTATCATACAGAAAATGCTTATG20.11757789535567313No Hit
GTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAATCAAGAAAATTGC20.11757789535567313No Hit
GTTCATGCTCATGCCCAAGCAGAAAGTGGCAGGCCCTCTTTGTGTCAGAA20.11757789535567313No Hit
CCTCATGTCTGATGTTCTTCCCTCTGCATTCCCAGTGAATGCTGCCATGA20.11757789535567313No Hit
CTCTTCATATCCCTCATGCACCTTTAATTTCAATGTTTGCAGATTGCCCG20.11757789535567313No Hit
GCACCAAACGGTCTTACGAACAGATGGAGACTGATGGGGAACGCCAGAAT20.11757789535567313No Hit
GCTTACAGGTTCGATAAAACCTGTCAACTCCAGCTTGAATTCCTGCATAG20.11757789535567313No Hit
GCATCCTTTCCGAGAACTGTGAGTCTCTTAGTGGTCTTGTTGTAATTGAA20.11757789535567313No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA20.11757789535567313No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGT20.11757789535567313No Hit
GAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGG20.11757789535567313No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC20.11757789535567313No Hit
CCTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG20.11757789535567313No Hit
GCTGTTTGAAATTTCCCTTTGAGAATGTTGCACATTCTCTCATAAGCAAT20.11757789535567313No Hit
GAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTTAATGGGAA20.11757789535567313No Hit
ATCCAGATTGAATCGGTCAGCTCGCATGCCTTGTTGAACTCATTCTGGAT20.11757789535567313No Hit
GCTCCATAGCCTTAGCTGTAGTGCTGGCCAGAACCATTCTGTTCTCATGT20.11757789535567313No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC20.11757789535567313No Hit
ATTTGAAGTATTTCAATGATTCAACAAGAAAGAAAATTGAAAAAATCCGG20.11757789535567313No Hit
CTCTAGTTCCTAAGGCCATTAGAGGCCAATACAGCGGGTTTGTTAGAACT20.11757789535567313No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA20.11757789535567313No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20.11757789535567313No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA20.11757789535567313No Hit
GGTACTGGCAGAACTGCAGGACATTGAGAGTGAGGAGAAGATTCCAAGAA20.11757789535567313No Hit
GGAACATCCCATCCATTCAATCCAGAGGTTTGTTTGGAGCCATTGCCGGT20.11757789535567313No Hit
ATTTATGTCTTACTTCTTCAATCAGCCATCTTATTTCTTCGAACTTCTGA20.11757789535567313No Hit
GTATGATGTTCTTATCCATTATCGCCTGGTCCATTCTGACACAAAGAGGG20.11757789535567313No Hit
ACCAAAGAGTATCCCTCTTTTTCGAAGTCGTACCCACTGGCTACGGCAGG20.11757789535567313No Hit
CAGTGACACACTCTGTCAACCTACTTGAGGACAGTCACAACGGAAAACTA20.11757789535567313No Hit
CTACTGGACTCTGCTGGAACCCGGGGACACAATAATATTTGAGGCAAATG20.11757789535567313No Hit
GGACAAATGGAACCTCAAAAATTAAAATGAAATGGGGAATGGAGATGAGG20.11757789535567313No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA20.11757789535567313No Hit
GAATGATAGATGGATGGTATGGTTATCATCATCAGAATGAACAAGGATCT20.11757789535567313No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT20.11757789535567313No Hit
CCTGTAATCTTTGATGAATAACTGAAGGGCCATTTGAGCAGTTGCTGGGC20.11757789535567313No Hit
TGCCTGGCCTGGCTAGCAACCTCCATGGCCTCTGCTGCCTGCTCACTCGA20.11757789535567313No Hit
GAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGA20.11757789535567313No Hit
GTAGGAAAAAGGGCAACAGCTATACTCAGAAAAGCAACCAGAAGATTGAT20.11757789535567313No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC20.11757789535567313No Hit
GAAATAAGATCCTTCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCG20.11757789535567313No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCCGCAAACAAGT20.11757789535567313No Hit
GTAGAAACAAGGTCGTTTTTAAATTATTCGACATTAATTGATGGCCATCC20.11757789535567313No Hit
CTCCCATTCTCATTACTGCTTCTCCAAGAGAATCTCTGTAGAGTTTTAGA20.11757789535567313No Hit
AATTAAAGGTGCATGAGGGATATGAAGAGTTCACAATAGTAGGAAAAAGG20.11757789535567313No Hit
ATGCTAGACGGGTGATGAACACCCCATAGTACAAGGACTTCTTTCTCTTT20.11757789535567313No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA20.11757789535567313No Hit
GCTCAAAACTACTCTCTGTTATCTTCAATTTATGTCTTACTTCTTCAATC20.11757789535567313No Hit
GGTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGA20.11757789535567313No Hit
GAATAAAAGAGCTAAGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA20.11757789535567313No Hit
AGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACG20.11757789535567313No Hit
GGCAATGAGAGCCATTGGGACTCACCCTAGCTCCAGCGCTGGTCTGAAAA20.11757789535567313No Hit
TAATTAAGCTTTGATCAACATCATCATTCCTCACTTCTCCACCTGGAGTG20.11757789535567313No Hit
CCCCTTATGGGAGCATGATGCCGTTACTCCTTTGGTTACGGTGTGGTTGG20.11757789535567313No Hit
CAATAGGAGAGTGCCCAAAGTATGTCAGGAGTACAAAATTAAGGATGGTT20.11757789535567313No Hit
GAACAATGGTGTTGGAGTTGATCAGGATGATCAAACGTGGAATCAATGAC20.11757789535567313No Hit
TACCATACCTGTATGAAAACCCCTTTACTCCGTCTGCTCCATCAACAGTT20.11757789535567313No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)