FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAAACCAGCCAAGTGTACAGCCTAATCAGACCAAACGAGAATCCCG14.3478260869565215No Hit
ACCTAGAACCGCATCCCAGGGCTGGGAAGGACACTAAGAAAACAGGAGGA14.3478260869565215No Hit
GGTCATGATCCCAGCGTCCTAGGATCAAGCCCCACATGGGGGCTCTCTGC14.3478260869565215No Hit
ATCCAGATTGAATCGGTCAGCTCGCATGCCTTGTTGAACTCATTCTGGAT14.3478260869565215No Hit
CATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCA14.3478260869565215No Hit
AATTGATGGCCAACCGAATTCTTTTGGTCGATGTCTGGCTGTCAGTAAGT14.3478260869565215No Hit
CATTTGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATT14.3478260869565215No Hit
GGGGATTTCGCCGCCGATGAGGAAGAGAGGGTGCAATGGAGTTCAGTATC14.3478260869565215No Hit
ACCTCTTACTTTGGGTCTTTTTGCTATTTCTGGGGTGAATCTCCTGTTAT14.3478260869565215No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACGTCAGTGCATGTGTGAGGA14.3478260869565215No Hit
ATTCGGATTTATTCCCTTTCCGTGTGGTTTGACAACAGAGGGTTCTTTCC14.3478260869565215No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT14.3478260869565215No Hit
CCCTAGCTCCAGCGGTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG14.3478260869565215No Hit
CCCATGGATCATGACCTGCCTGTAAGGCCCATCTATATAAAATGGTAGGA14.3478260869565215No Hit
CTACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGC14.3478260869565215No Hit
CCAGAACAACATAAGAGTTAAAGAAGCAGAGGGAACAATCCCAAAGAAAC14.3478260869565215No Hit
GCGCGATTCGGCCCAACTGACCATCGTCATAATCCACATCCTGCGTCTGA14.3478260869565215No Hit
GAAAAAAGGAGTTTTAATCAACGGAATACAAGTCAATTGTGAATCGCAAC14.3478260869565215No Hit
CCCTTATAGAATTAAAATTGGACCCAGATTTCTAAAGATATGGGATCTGG14.3478260869565215No Hit
CAAGGGTATTTTTCATTAATTGTCGTACTCCTCTGCATTGTCTCCGAAGA14.3478260869565215No Hit
CTTCTTCTGGTCTTGCACTTTCCATCATCTTTATGATTTCTGCCCTCATG14.3478260869565215No Hit
AATATGGACATAGCCGTTAAACATTATAGAAAGCCGAATAGCGAGATAAA14.3478260869565215No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG14.3478260869565215No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)