FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_E04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_E04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG23.4482758620689653No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.7241379310344827No Hit
GATGAAATGGGGCATGGAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGC11.7241379310344827No Hit
CCTTTTGCAAGATTGCTCAGTTCATCGTTTGTTTTGCTCCAGAGGGTTTG11.7241379310344827No Hit
CAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGC11.7241379310344827No Hit
ACAAAAACCACCGTAGACCACATGGCCATAATCAAGAAATACACATCAGG11.7241379310344827No Hit
AGCAGGTACTGGCAGAACTGCAGGACATTGAGAGTGAGGAGAAGATTCCA11.7241379310344827No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT11.7241379310344827No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.7241379310344827No Hit
ATCAATGAGCAAGGCGAGTCAATAATAGTAGAGCCTGAAGACCCAAACGC11.7241379310344827No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT11.7241379310344827No Hit
GGCAGGAGAAGTTCGGCGGGAGGCTTTGGTCAGCGAGCCTGCGCATTGTT11.7241379310344827No Hit
GTTTTGGAGTAATTTGAATGGGTCTATCCCGACCAGTGAGTACCCTTCCC11.7241379310344827No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA11.7241379310344827No Hit
GTACTGATCCAAATTGGAAGAGTTTGTGCGACAATGCTTCAATCCAATGA11.7241379310344827No Hit
TTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAG11.7241379310344827No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAG11.7241379310344827No Hit
CCTATAGGACAGCTCATTAAGGCCCTATAAGGGCTTCTGTCCTTAACGGT11.7241379310344827No Hit
GAACCGAATGGGTCTGGCCAGCACTACAGCTACGGCTATGGAGCAAATGG11.7241379310344827No Hit
ATATAAAAGGTTCTCTTATGACAAAAACATCTCCTTTGGAACCAATTCTT11.7241379310344827No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11.7241379310344827No Hit
CCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTG11.7241379310344827No Hit
CCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGAAAACTCCATGCTC11.7241379310344827No Hit
TTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCA11.7241379310344827No Hit
AGGGAAAAATTTCTCGAATAGATTGCAGAACTTCTGTAAGATCTGATCAT11.7241379310344827No Hit
TTGATGAAGAGTAAGTTATTGTTAGTTTCTCTGTTCCCTGTGTTTCACTG11.7241379310344827No Hit
GGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAATGAAAAGATGT11.7241379310344827No Hit
CCACTAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAG11.7241379310344827No Hit
ATACTATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATC11.7241379310344827No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT11.7241379310344827No Hit
CCTTGGCCCTGAGGTCGGCCTGGCCCGGATTGATATCGCCTCGTCGGCGG11.7241379310344827No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC11.7241379310344827No Hit
GAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCTGTTTAATGTCC11.7241379310344827No Hit
ATACTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATA11.7241379310344827No Hit
GTGTGGATGGCATGCAATTCTGCTGCATTTGAAGATCTAAGAGTATCAAG11.7241379310344827No Hit
CATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCA11.7241379310344827No Hit
ACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAG11.7241379310344827No Hit
GATTAGGCTGTACACTTGGCTGGCTTGAAGCAGTTTGAAAGGGTCTATCC11.7241379310344827No Hit
CTATCCATACTGTCCAAAAAAAGTACCTTGTTTCTACTCTGTCTCTTATA11.7241379310344827No Hit
AGCTGAGTCCTCTGTCAAGGAGAAAGACATGACAAAAGAGTTTTTTGAGA11.7241379310344827No Hit
CTATCGCAGTCCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTAT11.7241379310344827No Hit
CAGTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACAT11.7241379310344827No Hit
ACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAA11.7241379310344827No Hit
ACTCCATAATTACAAAGTTCCCCACAAGAGTATCATGTCTGAAAAGATAC11.7241379310344827No Hit
TGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAG11.7241379310344827No Hit
CAATAATAGTAGAGCCTGAAGCCCCAAACGCACTTTTGAAGCACAGATTT11.7241379310344827No Hit
AGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTA11.7241379310344827No Hit
GCTCGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTC11.7241379310344827No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATATCAATCGGG11.7241379310344827No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.7241379310344827No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC11.7241379310344827No Hit
TGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCT11.7241379310344827No Hit
GTATCAATAAGAGATAATCAAAAATAAGACAAAATAAACAATTATCACTG11.7241379310344827No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.7241379310344827No Hit
GCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGC11.7241379310344827No Hit
CACTATCAGTTGAATCAATCTTCTGGTTGATTTTCTGAGTATAGCTGTTG11.7241379310344827No Hit
GAATAAATATCTGGAAGAACATCCCAGCCCGGGGAAAGATCCTAAGAAAA11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA50.0145.05
TCCTCAA50.0145.06
TTCATCC50.0145.02
TCAAGAA50.0145.09
CATCCTC50.0145.04
GTTCATC50.0145.01
TTCCTGT50.0145.0145
CCTCAAG50.0145.07
TCATCCT50.0145.03
CTCAAGA50.0145.08
Produced by FastQC (version 0.11.5)