FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCTTAGCTGTAGTGCTGGCCAGACCCATTCTGTTCTCATGTGTTATT12.7027027027027026No Hit
GGTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACAGGATCGGGGC12.7027027027027026No Hit
CATATAGACTGTTGAATACAGATTTAGCCAGTAAGGACCTCCACACTTAC12.7027027027027026No Hit
ATTTTGGACAGTATGGATACCAAATAGTACCATTGCCACAACTACATCAG12.7027027027027026No Hit
ACCTACTGCTGTGAACTGTGTATTAATCTTTTCAATAACAGAATTTACTT12.7027027027027026No Hit
GTCATAAAGGACAAGTTCGCTCATCCACTTTCCATCTACTCTCTTGTATA12.7027027027027026No Hit
GTTCAGAAATATAATAAGTATAGCTCCAATAATCTACTCAAACAACAAGG12.7027027027027026No Hit
ATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAAAGG12.7027027027027026No Hit
GAGGAGAAGATTACAAGAACTAAAAACATGAAGAACACGAGTCAGCTAAA12.7027027027027026No Hit
ATGGGGTTCAGTAGCTGGTTTGCCCTATTGACAAATTTCAGATCGCCACT12.7027027027027026No Hit
GGCTCTCACTCTTCTCTTTCTCTGAAAATGAGTTGTGATCTCTACTGCTT12.7027027027027026No Hit
TCTCATTGCTGATCCACTCACATTCCCAGTCAATGAGGAGAATTGCATTC12.7027027027027026No Hit
GTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATCAGATCAGAATG12.7027027027027026No Hit
GTCTGTTATCTTCAATTTATGTCTTACTTCTTCAATCATCCATCTTATTT12.7027027027027026No Hit
GCTTCAAGCCAGCCCAGTGTACAGCCTAATCAGTCCAAACGAGAATCCCG12.7027027027027026No Hit
ACCCACGGGAGGTGGTTACGCGAGCGCGATGATGACACACTGACAAGCGG12.7027027027027026No Hit
GAACAGGATACACCATGGACACAGTAAACGAGTAATCGTTCTATACTCAA12.7027027027027026No Hit
TAATGAACCAAGTGGGTATGCACAAAAAGACTGTGTTCTAGATGCTATGT12.7027027027027026No Hit
ATCCACTTGTTCTCTTAAATGTGAACCCGCCAAAACTGAAGGATGAGCTG12.7027027027027026No Hit
GCCTGCCCCATCTGCCTGGCCTGGCTAGCAACCTCCATGGCCTCTGCTGC12.7027027027027026No Hit
CAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCA12.7027027027027026No Hit
GATAATCGCTCACTGAGTGAGCAGAAAGGCAAGCCCGCCCGAGGCACTAA12.7027027027027026No Hit
TCTCCATTCCCCATTTCATTTTAATTTTTGAGGTTCCATTGTGCGATCTC12.7027027027027026No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATAAC12.7027027027027026No Hit
ACTACATCCTGTTTCACTAAGAATTTACTATCGGTATCTGTCAAACCATT12.7027027027027026No Hit
GAGTGCTATTTCTTTGGCCCCATGGAATGTTATCTCCCTCTTAAGCTTTC12.7027027027027026No Hit
AAACAAGAGGATCACTTGAATCGTTGCATCTGCACCACAATTCGTTTCTG12.7027027027027026No Hit
GGATGAAATGGATGATGGCGATGAAATATCCAATTACAGCAGACAAAAGG12.7027027027027026No Hit
GAGTATCCCTCTTTTTCGAAGTCGTACCCACTGGCTACGGCAGGTCCATA12.7027027027027026No Hit
CACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTATCAAG12.7027027027027026No Hit
GGGCGGCAGGGGTGTGGTGTTGGGCGCGCTCTGGTTCGCGCAGCGGGTCG12.7027027027027026No Hit
GTCCGCACTGGCGTCATCTAGCCCCCCAAGATAAAAGGTCCCAGTTTCCT12.7027027027027026No Hit
GTAAACAGTATTTGCAACACCACAGGAGCTGAGAAACCAAAGTTTCTGCC12.7027027027027026No Hit
GAAATAGCACTCAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCT12.7027027027027026No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG12.7027027027027026No Hit
GGGCCATGTTTCTGATCTATTCTCAAAAAACTCTTTTGTCATGTCTTTCT12.7027027027027026No Hit
GCTCTATGCTGCCAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)