FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTCTCCCAGCCTGCCTGCCCTGGGCTGGATGGCCCCCCTACACCTCT13.125No Hit
GTACAAGAATGAACAGTAGACAGATAATCTCGACTACATCTTGATCACCG13.125No Hit
AATCTAGACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGG13.125No Hit
CCTCAGCATCCCTGCTTGCATCTGCTCTATTTTAATAGTTACAAATTATG13.125No Hit
CCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGACGCGCAGGCTGGGAGA13.125No Hit
AACTGCAGCTGCGACCATGTAACTCATTTCTAAGTAGTAAGTCAGTACTT13.125No Hit
GGGCTGTTGCCCCCTCCCCTGCCCATTATCCTGGAAACCACGGATGCTGG13.125No Hit
CTACCACACTGCCCCTGGTCGCTTAATAGGTGTCGTCCTGGGTTTAGGTC13.125No Hit
GAATAAGCCAAACTCAAGTTTCTTTTTCTTTTTTTTTTTAAATATGGAAC13.125No Hit
CCTTTTAACACTTGCTTTAGAAAATGATGCGAGGGGCCAGGTTGCGCGGG13.125No Hit
CGTCAAAGACAGTTCCGCCTTCTGCCTATCCCAATGGCCCAGGAAAGGAC13.125No Hit
CCCCTGAGGGTCACAGACTGACATTGAAGTCGCTCCCTGCCGCATATAGA13.125No Hit
GGCAAGTACCTAGGAATAAACATAACCAAAGAATTAAAAAATCTATACAC13.125No Hit
TCGGTGGTGGCAGCACTATCCTTCTGACCCACAACCAAGTTAAAGGCATT13.125No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA13.125No Hit
ATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACT13.125No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT13.125No Hit
CTCCTAAAATGTGATAAAATTATATGACAAATTAGGAACTGGTTATGATG13.125No Hit
ATCTCCACTTGTTTGTTGAACAACTTCAATTGTTTCAAGGCATGAATTCT13.125No Hit
GGGCCTCAGTGCATGGTGCCCTGGGAGGGGTTTTTCTCATGCCCGGCCAG13.125No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC13.125No Hit
ATTTTAAAATGAACAAATATTTAATTATTAAATACACAGAATAAGGAAAC13.125No Hit
TGATAGAAAAGTAGCTCCTTAAAAGCCAAATGCTAAAAAATCAGTGCCTT13.125No Hit
TCTTTAACTTGCTCCTGATTTTTCCTGCAGAGTGATAAGTGCCTCTTCAG13.125No Hit
GGATAGGACTTGTTCCATCTGGTTTTGATAAAAAATATTTTAGTATCTTC13.125No Hit
CAATACGTGCCCTCCACAATTCCCGCTACCAGACTCACCTAACCCCCCCC13.125No Hit
GCCCAGGATCCAGCCCTGGATGTCTCTCTGCTGTCCGCGGGTCGGGCTGT13.125No Hit
ACAGTGGACAATATGGCAATAATAAAGAAGTACACATAAGGAAGACAGGA13.125No Hit
TCCCTGTACTGACCCTGACCCTCAAGAAAACTTAACAACCCTCAAGAAAA13.125No Hit
AAGAGGGGGTCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGA13.125No Hit
AATATGTCCCTGTTAACCGTGAGAGGGATATCTGGTAGGCACGGGGAGGT13.125No Hit
GGATAGACATCTTAACAATATTGTTCTTCCAATCCATGCGCATGGATTGT13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)