FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA70.3015941404566997No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA60.25850926324859974No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA60.25850926324859974No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA50.2154243860404998No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50.2154243860404998No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT50.2154243860404998No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT50.2154243860404998No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.17233950883239982No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.17233950883239982No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.17233950883239982No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.17233950883239982No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.17233950883239982No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG40.17233950883239982No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT40.17233950883239982No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.17233950883239982No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.17233950883239982No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG40.17233950883239982No Hit
CCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTC30.12925463162429987No Hit
CTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTC30.12925463162429987No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.12925463162429987No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA30.12925463162429987No Hit
TCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCG30.12925463162429987No Hit
CTGCAAAGATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGC30.12925463162429987No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.12925463162429987No Hit
TCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACA30.12925463162429987No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA30.12925463162429987No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC30.12925463162429987No Hit
TTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGT30.12925463162429987No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG30.12925463162429987No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.12925463162429987No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT30.12925463162429987No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.12925463162429987No Hit
TCACTGACTTCTTCGGGAGACAATAGTACGTTCCCTCTTTGATCTCTAAC30.12925463162429987No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA30.12925463162429987No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG30.12925463162429987No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.12925463162429987No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.12925463162429987No Hit
CATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCA30.12925463162429987No Hit
CCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGG30.12925463162429987No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT30.12925463162429987No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.12925463162429987No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA30.12925463162429987No Hit
CTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCTGTAGAATCTGT30.12925463162429987No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA30.12925463162429987No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC30.12925463162429987No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.12925463162429987No Hit
GAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAA30.12925463162429987No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30.12925463162429987No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT30.12925463162429987No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.12925463162429987No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.12925463162429987No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT30.12925463162429987No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA30.12925463162429987No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.12925463162429987No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)