FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAGTTCTAAGGCTTGCATAAATGTTAATTGTTCGAAACTATTCTCAG15.555555555555555No Hit
AAACAAGGTCGTTTTTAAACAATTCGAAACTAAATTATTGCAATACTAAT15.555555555555555No Hit
ACAATAAGCAGCAAAAGCAGCAGCAACATGGGGAGAACGTTGGCTCCGCG15.555555555555555No Hit
GTTTTCAAAGAATTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGG15.555555555555555No Hit
TTCCCATGCTTATGACCCTGACTATTAGTGTCTCTGGCCAGCGGGGAGAA15.555555555555555No Hit
CTCCTATATGAACTGGTAGGGAAAAATTTCTCGAATAGATTGCAGCAGTT15.555555555555555No Hit
CGCGACAATCACTAAGACCACTGTGTCCCATATGGCAAGAATAAAAAAGT15.555555555555555No Hit
CTTCTGCCCTCAGGGACTCAGGCCCTCCTTCCTTAGAAGGCACTCTTTTA15.555555555555555No Hit
GATCATTTGAGTTAAAGAAGATTTGGGATCAAACCCAATCAAAGGTAGGG15.555555555555555No Hit
GATTAGGATTCTCTTTTGATTTGTCTCCCTCCCGATCCCCACATTGCTTC15.555555555555555No Hit
GTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAA15.555555555555555No Hit
GGCGCTAGGATGAGTCACAATAGTGCTCATTGCATGTACCATATGCCTAC15.555555555555555No Hit
GCACCGGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTCTATATG15.555555555555555No Hit
CTCTAAAACCCCTGCCTACTGCCTCTCCCCAGGGGACCCGGACAGGTCCA15.555555555555555No Hit
GCTCCATGAGAAAATTATCCACAAATCCAGAGCGAGAAAACAAGCGTTTG15.555555555555555No Hit
CCCCAGTGAGCAGGGCGCCCGATGCGGGGCTCAATCCAGGAACCCGAGAT15.555555555555555No Hit
CCTAAGGTCCTGCACACTTTCCCAATAGATCCTTCATCCCCTCCCCTGGG15.555555555555555No Hit
ACTTCTACCTGATTATCCAAAAGAAAAAAACGCTGGGCTTTTTTCACCAT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)