FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753966
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9270.12294984123952539No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC9160.12149088950960654No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA8850.11737929827074431No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA8630.11446139481090659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2950.056.524319
AGCGAAA2000.047.1303251
GACAGTA3050.045.1630487
TAGAAAC6900.044.135423
CGAAAGC2300.044.1354183
ACGGGCG700.00105623341.430508145
GTAGAAA7250.041.004632
TATACGT1303.6934762E-739.0428734
AGTAGAA6900.037.830361
GGTCGTT2252.0008883E-1135.443757
GCGAAAG3000.033.8371542
ACTGATC4450.032.583638
ACAATTC5200.032.0666778
AGGTCGT2558.731149E-1131.2780446
GTCTAAG3400.029.8563141
GTATTAC6850.029.6383841
GGATAGC4250.029.0032755
CGTTCCG1555.4969045E-528.065825145
TATATTC6300.027.6221683
TTACGCA1050.007703019327.6221684
Produced by FastQC (version 0.11.5)