FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTAGAACAAATAATCCTAAAATTTGTATGGAACCACAAAAGAGCCTG22.3529411764705883No Hit
GAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGA11.1764705882352942No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11.1764705882352942No Hit
GGGGTGGAGTCAGCTGTCCTGCGAGGATTTGTCATTTTGGGGATAGATGA11.1764705882352942No Hit
CGCACAGGCGGGAGGTGCTGGGGACCTGTGCCACTGTCCACATAAGAACC11.1764705882352942No Hit
CCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCT11.1764705882352942No Hit
GTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTATATGCGTCTC11.1764705882352942No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT11.1764705882352942No Hit
GGCCACTTGGTATCCCTTAAGGCTTCACTCTCCCCTCTCAGCTCCCCAAG11.1764705882352942No Hit
TACTTGTTCTGAGGGCTCGAGTGAGAAATTGCTTTCCCCGTTCCCCATCT11.1764705882352942No Hit
GCTGCACGTAACAAATTATGGACCCTACATGGTGCCGATGATTGTACATT11.1764705882352942No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC11.1764705882352942No Hit
AATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTAC11.1764705882352942No Hit
GTCACAGTGCTGTTGTTTTTCAGGGGAGAAGACATTCTCAGCATCCTTAC11.1764705882352942No Hit
AATAGTGGCAGGGTGAGTCCCCAGGTGGGGATGGGCTGGACCTGTCCGGG11.1764705882352942No Hit
GTTTTGCCCTGCAACCTCAGTTCTCTGACAGATCCAAGAAGAGTAGTTGA11.1764705882352942No Hit
GGGTGGCTCAGTGGGTTAAGCCTCTGCCTTTGGCTCAGGTCATGATCTCA11.1764705882352942No Hit
GTCCCCCCACCCTGCCTCAAGCAGGGGGTCCCATGGTCAGATGGCCCCAG11.1764705882352942No Hit
ATATATTACTGTTGGCTCTGTCTGGTGCTGATGGATTAGGTAGTTTTTAT11.1764705882352942No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG11.1764705882352942No Hit
GGGAGTTCCTGGCTCGCTGCAGAGCTGGGCGTCGCTGGGGACTGGCGCGG11.1764705882352942No Hit
GGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGCTGCGGAGAAGG11.1764705882352942No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT11.1764705882352942No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.1764705882352942No Hit
GCCAGGGGGTGAGGCTCAGCCTTCTCTCGAGGAGCTGGTGGGTGCTGAGC11.1764705882352942No Hit
GATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCAC11.1764705882352942No Hit
ACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGT11.1764705882352942No Hit
ACCGAACAGTGCTGGTGATTGCTCACAGGCTGCAGGCGGTGCAGAGTGCG11.1764705882352942No Hit
GGTCAGAACTGGCGCCCAAAAGGTGGGCCCACGCTCCTTGGTAGCTAGGG11.1764705882352942No Hit
GCATTTCATTGATGTAAACTGAGCGCAAGGTACTCTGACATCCAGACCAT11.1764705882352942No Hit
GAGGTGGCCCTAAGCTATTCCCAGGGGGCACTTGCCAGTTGCCTGGGCCG11.1764705882352942No Hit
CTACCTGGCTTTGCATGTTCGAGGAAGAGCGCAGAGTAAATGCGAATGGA11.1764705882352942No Hit
GAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGA11.1764705882352942No Hit
ATATGGAGAGAATAAAAGAACTGAGATCTAATGTCGCAGTCCCGCACTCG11.1764705882352942No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11.1764705882352942No Hit
CTTAACCCACTGAGCCACCCAGGCGTCCTGGACCTGGAATTTTTAACTGA11.1764705882352942No Hit
GAAATACATGTATTTGTAGGTGGTGGCAGAGGGGGCATATACATGCCACT11.1764705882352942No Hit
CCAAGACTCAGAAAAGGGAAAGTGGACGACAACACCAGAGACTGGTGCAC11.1764705882352942No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA11.1764705882352942No Hit
TCCTTATTCTTCACTCTTTGCCTTCTTCCCAGTTTTCATCTGGCTGGTTT11.1764705882352942No Hit
CTCCCAGGGGGTCCTCCTGGGTGCCGATCACATAGATGTCATGGGGGATG11.1764705882352942No Hit
TCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTA11.1764705882352942No Hit
GCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACC11.1764705882352942No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT11.1764705882352942No Hit
GTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGC11.1764705882352942No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA11.1764705882352942No Hit
GGCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGCCAATTGAA11.1764705882352942No Hit
ACCAAACACTGGGAGCAGAGCCCAGCACTCTGTTTTAACGAGCCCTCCCT11.1764705882352942No Hit
GTTATTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC11.1764705882352942No Hit
GGAATAGGAGCAGGAGCAGGTGCAGGAGCTGGATCAGGTGCAGGATGAGG11.1764705882352942No Hit
GGACAAATCTTCTGGCTTTGCACTTTCCCTCCTTCTTATAACTTCTGTTC11.1764705882352942No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT11.1764705882352942No Hit
GCTTCATGGAGTAATTTTACCCTTCTTGGTAAAGTTGCAGAGCTTTGAAA11.1764705882352942No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGAAAAA11.1764705882352942No Hit
CTCCATCTCCGGGCTCTACTTCCCTTCCTTCGAGGCCGGCGCTCTAACCA11.1764705882352942No Hit
GTTGAGTGGTGAGGTTGGCATCACAGTCCCCCATGATTGTGACAGAAATA11.1764705882352942No Hit
CTAGATAGTAGCCTGGGATTCTGACCCAGATTTCTTAGCAACAGACTTCA11.1764705882352942No Hit
TTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGT11.1764705882352942No Hit
AAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCT11.1764705882352942No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC11.1764705882352942No Hit
AACTTATTCGTCATCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGC11.1764705882352942No Hit
CCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCCTCC11.1764705882352942No Hit
CTCATAAGTTCGTTTGCACTACCGATTGGTGCGATGTGTTGAACCATGCG11.1764705882352942No Hit
GTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG11.1764705882352942No Hit
GTCCCGTTTTCGTTTCATTACCAACCCTACGAACCCGTGCCCCATTACCA11.1764705882352942No Hit
CTTCAAAGATTTCTGCTATTAGAATAAGGTTCGGTTTAGTTAAGGTGTCA11.1764705882352942No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA11.1764705882352942No Hit
CCGTGAGGTACGTCGCATCAGGTCCTCGAGCGCAGGGAGCAGAGCACAGT11.1764705882352942No Hit
ACCTCGCATGCTGTGAGGAAGATCTGAGCAGAAAGGAGGGAAGAGCTTGA11.1764705882352942No Hit
GGATGCGGACCAAACAGTCTGCAATTCGGAAGCCAGGAAGATAAGCAGTC11.1764705882352942No Hit
CAGTTAAACAATGTGTGCAAATCATCTCGATTTTCACAATTCTGTGGGGT11.1764705882352942No Hit
CTAAACTTCAGATCAAATCCTGTAAAGCAACACTGTATCGCATGCATAAG11.1764705882352942No Hit
CTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCC11.1764705882352942No Hit
GACGATGGGCTGCAGACAGACGGCAACCGCTGTGGCCACTTCCTGGCGAG11.1764705882352942No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAAGTTTCGCGTTC11.1764705882352942No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT11.1764705882352942No Hit
GGCAATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAGAGAAGAGAGC11.1764705882352942No Hit
GTGTATTATATGGCCATTCAGGAGCCCTGCTTTCCAAAAACAGGTGTCAG11.1764705882352942No Hit
AGCCAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGGCAGCA11.1764705882352942No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGCCCCTGGCATCAATCCGGGCCCT11.1764705882352942No Hit
AGTTTGAGTCTAGCTCCACAGCTCCCTGATGCAGCAGGGACCCATCAAAA11.1764705882352942No Hit
CATAGTTCCTGTAATCTCAAATTTTTCTTCAATTGTCTCTTCGCCTCTTT11.1764705882352942No Hit
GAGACTGGTGCACCACCGCTCAACACGATTGATTGACCACTCCCTGAGGA11.1764705882352942No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC50.0145.08
ACTTGGT50.0145.05
CGCTTGC50.0145.0145
CTTGGTA50.0145.06
CCACTTG50.0145.03
CACTTGG50.0145.04
GGCCACT50.0145.01
GCCACTT50.0145.02
TTGGTAT50.0145.07
GGTATCC50.0145.09
Produced by FastQC (version 0.11.5)