FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATTTGCTA17.142857142857142No Hit
TGACTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA17.142857142857142No Hit
AACTTATACGATATACGGAATCTTCAAATTCCTGAAGTCTGCTTAAAATG17.142857142857142No Hit
GCATAGTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTT17.142857142857142No Hit
AACTAGGTGTCAGGAGTGAGGCCCCCTGGGGTCAAGGAACCAAAGGAGGG17.142857142857142No Hit
GTATGACGCCATGGGCATTAGCTGCCTGGGCAGCTGCCATCACCTTCTCA17.142857142857142No Hit
GAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGTAAACATGGCC17.142857142857142No Hit
GTTATCTCTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAA17.142857142857142No Hit
CCATTGAATTTTCACACTTTCCCAGTTCCTGATTATCCATTGATAAGTGT17.142857142857142No Hit
CCCCCAAGCAGGTCCGTCTCCAAGGAGTTCACCACGGAGTTTTGAGTCAA17.142857142857142No Hit
GGTGGAAGAGACAGACATCCTGGAGGTGTTGCCGATACTAATAGTTGTTG17.142857142857142No Hit
CTCCCTGGAAGCCCTTCCGGGTCAACATGTGCATGGACACGGTCCTCACG17.142857142857142No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA17.142857142857142No Hit
CGTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCT17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)