FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512671
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA23400.45643307306245134No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21320.415861244345789No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA13550.26430205726479555No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10210.19915306307553968No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9710.1894002196340343No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG9690.1890101058963741No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8300.16189720112898914No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG8030.15663066567057626No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG7980.15565538132642573No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC7550.1472679359667311No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA6940.13536946696809457No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5960.11625389382274402No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC5910.11527860947859347No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5220.10181968552931608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG5750.054.216489
GTCAATT2750.052.736672
ATTATAT7650.052.138561
TTCAATA7650.048.332527
TATATTC7900.047.7300263
ATATTCA8550.045.7976384
GTTACGC803.728163E-545.320583
TATTCAA8600.044.6881985
ACTATTC7300.043.697898
TTATATT8700.043.3410572
GGTCAAT3350.043.295521
CGAAAGC2200.042.8485452
TCAATAT8650.042.744958
AACTATT8050.042.328487
ATTCAAT8950.042.1304176
TTAAACT8700.040.007134
AAACTAT8200.038.9093746
TCAATTA3600.038.270713
AGCGAAA1157.1224076E-637.836521
TAAACTA8750.037.292365
Produced by FastQC (version 0.11.5)