FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCCCATCTCTCTCTCTGCCTCACAAGTTCCCTGGGTCTTCTGGGAA28.695652173913043No Hit
GTACTGTCATTTGCAAATATCTTCTCCCATTACAAGGGTTGCCTCATTGT14.3478260869565215No Hit
GCATATCGCCGCACATGTAAACCAGGGTCTGCACATAATGGCCCATGTCC14.3478260869565215No Hit
AAACAGCACTAATGCTTTACCTGTGTACGCTCAGCCCCGGGAGCCCCTTT14.3478260869565215No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA14.3478260869565215No Hit
CATCATACCCGCAGCACTTCCACAACCCCACGCTGCAGCCCTGCCAGGGA14.3478260869565215No Hit
AGGAAGACTCCTCCCTCTCAAGTCACAGCTCAAGCATTCTGGTGCCCCCT14.3478260869565215No Hit
GTAATGGGTGGGGGCGGGGAGGACAATTATTCTTTGCAGTTCACCTGTTA14.3478260869565215No Hit
GCTAGGAACAGCGCCCAAGGTCAGGAAGGAGGACAGTCCCGTGACCTTGG14.3478260869565215No Hit
TTCTACTGGAGGGGAGAACCGTTTTTTTTTTTTTAAATTTTTTTTATTTT14.3478260869565215No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTAGCA14.3478260869565215No Hit
ATATCAAAAGACACGGTCTACAGAGTAAAAGGACAGTCCACAGAATGGGA14.3478260869565215No Hit
GCATTTCAGAGACCCTTTAGTAATGGGTCTCCCACATGTCTGTGCTGACC14.3478260869565215No Hit
ACTGAGAATCTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTG14.3478260869565215No Hit
CTTCCACCATGCCAATAGCTCTAGCAACAACTACCCAGCAGCACAAGCCC14.3478260869565215No Hit
GTATTTCAGGGGCAAAGAGAGAATAGAGCAATTAGAGGACTACAGTAGAT14.3478260869565215No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAG14.3478260869565215No Hit
CGGCAGGCTGGTGTGCTGCACACGGACAGCTGGATGCAGAGCTTCTAGTT14.3478260869565215No Hit
CGGCCAAGCGAAGCCCGGTGTCGGCACCACTCCTGCGCCATCGCCCTCAT14.3478260869565215No Hit
CGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGCACATTTGACA14.3478260869565215No Hit
GAATTAACTGTCCCCACAATGCAGTGAGGTATGAAGCTTGATTTCTTGGC14.3478260869565215No Hit
CCTCAAGCCTGTCCTGCCTCACAAGGTATGGGTCCCTTCCTCTGCAGGGA14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)