FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_D01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_D01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019438
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA17760.17421363535595102No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15920.15616447493619035No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA11750.11525958420227617No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT11460.11241487957090084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG5950.049.9561429
AGCGAAA2250.041.9037861
TATATTC9400.040.890193
GGTCGTT4000.038.0610967
TATACGT2600.036.2611164
CGCCAAT4000.036.2611124
AGGTCGT4200.036.2611126
CGAAAGC2700.034.9181063
CAATATG10100.034.4541749
TCAATAT10450.033.3002058
ATATTCA10900.033.2670754
CGTGCTA3100.032.7407268
ATTATAT9050.032.0555951
GTCGTTT5050.031.5829938
GTAGAAA11550.031.3964421
ATTCAAT12300.031.2494146
TTAAACT11300.030.80594
CTCCGAC1652.3844368E-630.76097145
ATTCGAC7600.029.5828861
TATAGAC11450.029.1355692
Produced by FastQC (version 0.11.5)