FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAACTAAAGAGCCACCCAGGCTCACCAAGGTATTTGGTATTTAATGG13.0303030303030303No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA13.0303030303030303No Hit
TGATAATTGTCTGTCTCCGATTGACTATTTTCACTCAGCGTCATCCCCTC13.0303030303030303No Hit
GGGACTGCGAGAGGGAAAAGCAGGCTCCCTATGGCGCAGGGCGCCGGCTG13.0303030303030303No Hit
GCTCTAGTTTTGTTAGGGAAAGTGTATCCTGTAGAAGCTTGCTTTAGAAG13.0303030303030303No Hit
GCAAGGAGACTGATGCAGGAATGATTCCAGGACCTGAGCAGAAGGCAGTG13.0303030303030303No Hit
GGTCGGTCACCGTGGGATTTCCAGTACTGCCGAGCCTGCTCCCCCTGGAC13.0303030303030303No Hit
GGCCTTTTCCTTCTAATGTAAACACTGCATTTGGAATTGAAAAAACCCAG13.0303030303030303No Hit
ACCTAAGGTACACCATGGTGGCTATAGATGATAATACTGTATACTTGAAA13.0303030303030303No Hit
CATCAGGAAAGACTACTTAGGGGAGAAAGAACAATAATCACCTGATGTAA13.0303030303030303No Hit
GCCCCGGGAGCCCCTTTCTGGCTCGGCGGGCCTTGGGTTCTACAGCAGTC13.0303030303030303No Hit
GTTGCATTATAATTGAGTTAACATAGTCTAATAGAGAAGGAAGAGCTAAT13.0303030303030303No Hit
TCTCTCTCAAGGCCAACATCCCTGAGGTGGAAGCCGTCCTCAACACCGAC13.0303030303030303No Hit
CAGGAAACTGCTCTACTGGTTGCTCAAATTAGGCTTTGTTGATTCGAGCA13.0303030303030303No Hit
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC13.0303030303030303No Hit
GGTACAGATTCTGCAGCAAGGCAGCCACTGTGCTCTTCCCGGACACATTG13.0303030303030303No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC13.0303030303030303No Hit
ATTTCGGGCTCCGTAATGGGTTATGGAGGACTCTTCCAAAAGGAGAATCT13.0303030303030303No Hit
TATTTTATTTTTTTGACAGACAGAGATCACAAGTAGGCAGAGAGGCAGGC13.0303030303030303No Hit
GTATTCACTCTCGCTTTCAAACTGTGCCGGATTTCTTGAATTAACCACCT13.0303030303030303No Hit
GATCAATGCACATCTCCAAGCAGGAATCCTAATAGTCAACCAGAGAGTGG13.0303030303030303No Hit
ATAGTGGGGTTGCCAAGCGGAGGCCCTTAAGGGTCAGCAGCTGGAAGCCT13.0303030303030303No Hit
GCCCTCTAGGCACCTGCCTGCGTTGCTCTGAGCCTGTTATGTAACACCAG13.0303030303030303No Hit
TGTCTGGCCATTGCCGGGGCCCTTGTGCGGCACCCACGAGTCCGCCGCCG13.0303030303030303No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA13.0303030303030303No Hit
GTTCTTAGCCATGCTCTGTGTTATCCAGGCCCCACACCACCCCGCATGCC13.0303030303030303No Hit
TCCGGGCATCCCGCAGCTCTTCGGCAGAGAAGCGGACGCGCCTGACAGGG13.0303030303030303No Hit
GGGTCTGCACATAATGGCCCTTGTCCTCCTCATCCAACAGGAAGGAGCGC13.0303030303030303No Hit
GCATTTGATTGCAGCATATAGTGGTATTCCCTCCCCCTCGTGTCTCGGGT13.0303030303030303No Hit
CCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACGGT13.0303030303030303No Hit
CTGAAGAGCTGAAGTGATGAGAAGGTGATGGCAGCTGACCAGGCAGATAA13.0303030303030303No Hit
CTTTACCAGGAACTACATTGTTACTTGAGTGGTAAAAATGAGAAGGGTTT13.0303030303030303No Hit
ATATGGAAAGAATAAAATAACTAAGAAATCTAATGTCGCAGTCTAGCACC13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)