FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824456
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2500.049.309651
CGAAAGC3450.037.83123
GTACTAC1051.8513028E-434.530571
CATACCG950.004723695630.5304415
ATACCGA1306.476857E-427.8883826
TAGGCTT5400.026.855485
GCGAAAG4650.026.5089592
GTATTAA4950.026.36881
GTATAGT2559.46402E-825.5932451
CATATAC3902.0008883E-1124.17141
TTACGCA2802.3385292E-723.306724
GTTACGC2852.7739407E-722.8978313
GTATAGA6000.021.754261
TGGACCG2003.0995655E-421.752945
GTATTAC4801.4551915E-1121.1499731
TATAGTA5500.021.0937582
GCATAAG2806.077824E-620.7183421
TATATGA5453.6379788E-1219.9568234
CCTATTC4803.1286618E-1019.638073
TATAGAC3751.8046376E-719.3359452
Produced by FastQC (version 0.11.5)