FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284428
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATACACAGGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCAT27250.9580632005287806No Hit
CTCTGGAACAGAGCAGACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAA20760.7298859465312838No Hit
GTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACC17880.6286300926772329No Hit
AGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGCAATGAA17520.6159731109454766No Hit
CTCTTAAGCAGCACGGCCACATACACCCTTACTTTTGAATGTCACCGTCC16220.5702673435808008No Hit
GCCACATACACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTA15560.5470628770725807No Hit
GTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCTCTCTCTCTCTCTCT13770.48412955123968104No Hit
GTGGTACACTGAGAGATAAAAAGAGTGATAGAGCGAGGGACTGAGGGAAA13190.46373774733851797No Hit
GTTGAGTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTG11430.4018591699832647No Hit
CATACACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTT10920.38392844586327646No Hit
GAGTGGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAG10460.3677556358726989No Hit
GCATACACAGGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCATCG10420.3663493045691704No Hit
GAGCAGACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGC10260.36072397935505646No Hit
CTCCTGCAGTGGACGGTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGC10190.35826289957388163No Hit
GCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCG9640.338925844150365No Hit
GATCTGGGAGATGCAGTCTTTCTCTTAAGCAGCACGGCCACATACACCCT9630.3385742613244828No Hit
GATGGAGAGAATGAGGGATGAAAACTACAGATGGACAGATGGAGAGAATG9210.3238077826374337No Hit
GTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGT8400.2953295737409819No Hit
GATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTAT8260.2904074141786322No Hit
TTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGCA7790.2738830213621725No Hit
ATTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGC7630.26825769614805856No Hit
GTGTATATGCATGTATGTGTAGATGACCATATAGAAGTAGGAGTTTGAAT7560.26579661636688373No Hit
CATTAAGTGAGAGTCCATCCTCACCAGCAGAGTTGTCTTCTGCCACCAGT7480.26298395375982675No Hit
GCCTAATTGCAGATTGGGTCCCAATACCCACACGCCCTGACCCTCAAGAC7330.25771021137159494No Hit
GTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTGCCCCCATTCTTGG7180.2524364689833631No Hit
CTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTG7110.24997538920218826No Hit
GTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGG6840.24048265290337098No Hit
CCCTGACCCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATACGG6800.23907632159984252No Hit
AGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTA6760.237669990296314No Hit
GTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGT6750.23731840747043187No Hit
GTGTATGCAGAGTGAGTTGACACACCCACCTACTCAACTGCACAAACAAA6690.23520891051513915No Hit
GGGTTAGGGTGATGGCCAGCAGTGGATCTGGTTGACCGTCTCAGTTGTAA6530.22958358530102524No Hit
CCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCCTA6520.2292320024751431No Hit
GTCTTTCTCTTAAGCAGCACGGCCACATACACCCTTACTTTTGAATGTCA6400.22501300856455764No Hit
GTGTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGT6280.2207940146539722No Hit
GTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAACCCACA6050.21270760965868338No Hit
GAGTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTGCAG5980.21024652987750853No Hit
GTCTGGAACTATCAGGTATGCTTTGCTTGCTAGCATGACTGATGGCTGTC5490.1930189714092846No Hit
CCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTAC5440.191261057279874No Hit
CTACTAGGGTGAGTATCACAAGACCAAATACTGAGTTGCTATCAGTGAAT5350.18809681184693489No Hit
GTGTAATGCCCCCGCGGCCAAGAATGGGGGCAAGGACTGCGATGGCACCC5340.18774522902105278No Hit
GTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCT5340.18774522902105278No Hit
GCAGTGGACGGTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGCGGGAC5340.18774522902105278No Hit
GTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAG5300.1863388977175243No Hit
GCTTAAGTTAGCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATGC5220.1835262351104673No Hit
GTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGT5190.18247148663282095No Hit
TCCCAGAACAGCAATTGCCCCTTGAACCCACATTAAGTGAGAGTCCATCC5010.17614299576694278No Hit
GTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGT5000.17579141294106065No Hit
GAATGAGGGATGAAAACTACAGATGGACAGATGGAGAGAATGAGGGATGA4920.17297875033400367No Hit
GAGTGCACCCACTGGCGGAGGCGTGAGTGTAATGCCCCCGCGGCCAAGAA4900.17227558468223944No Hit
GTCCCAATACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGA4840.17016608772694672No Hit
GTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAGGCGTGAGTGTAATG4790.1684081735975361No Hit
GAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCGGAGCC4780.168056590771654No Hit
GTGCAGTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTGCCCCCATT4700.16524392816459701No Hit
AGCTTAAGTTAGCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATG4690.16489234533871489No Hit
GTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGTGT4640.1631344312093043No Hit
ATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCGGAGCCATGTT4640.1631344312093043No Hit
CTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCT4640.1631344312093043No Hit
CTTAGGGCCGGAGCCATGTTCTGAATACCAAGCACTTCTCATAAAAGGAG4550.1599701857763652No Hit
CTACAGTAGTGTTCAGGATTTCATAGTTTCATACATCTGGCCATACTTGA4510.15856385447283672No Hit
GTCTGAGGCTGGTTGAAGGGGTTAGGGTGATGGCCAGCAGTGGATCTGGT4400.15469644338813338No Hit
GTTAATTAACTGAATTGAAAGATTTTGGTTTCCGTGTGTGTCTGTGTGTT4380.15399327773636914No Hit
GGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTG4350.15293852925872278No Hit
GCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATGCTTTGCTTGCT4320.15188378078107642No Hit
GTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCTTCTTCTCTTG4300.15118061512931216No Hit
AAGCAGGAGAGAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAA4180.14696162121872672No Hit
GTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGT4150.14590687274108036No Hit
ACCAAATACTGAGTTGCTATCAGTGAATTTGACAGTGAGAGTGACTTATC4120.144852124263434No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGA4050.14239104448225914No Hit
TCTGAATACCAAGCACTTCTCATAAAAGGAGAACCTGTCCCGCATCCCCC4040.14203946165637701No Hit
GTGATAGAGCGAGGGACTGAGGGAAAGGGAGATGTCATATCTCCTCCCCC4030.14168787883049488No Hit
GTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGTGTCA3980.1399299647010843No Hit
ATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGAACCAA3950.13887521622343793No Hit
TTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCTCT3900.1371173020940273No Hit
GAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAATGAGGGATGA3860.13571097079049882No Hit
GTGGGGGCACTGTCCACCTGCTCCGCCTCTATGCTCTTCTCGCTCAGGCC3850.13535938796461672No Hit
GGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAGGCGT3830.13465622231285246No Hit
GGGGAGGAGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACT3780.13289830818344187No Hit
CTCTCTCTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGGAGTG3760.1321951425316776No Hit
GAGCAGAGGTGAGTGAGTGCAAGTGGCTGTTTGAGGTAACTGCGTCAGTC3750.13184355970579548No Hit
GTGGATCACTGCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCT3740.13149197687991337No Hit
GGACTATTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCC3690.12973406275050275No Hit
TACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAACAGAGC3690.12973406275050275No Hit
GGAGAGAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAATGAGG3650.12832773144697426No Hit
TACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAAC3640.12797614862109216No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTT3610.12692140014344577No Hit
GCAGGAGGGGGAGGAGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTC3590.12621823449168154No Hit
GGTATGCTTTGCTTGCTAGCATGACTGATGGCTGTCTGAGGCTGGTTGAA3560.12516348601403518No Hit
CCCAATACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAAC3540.12446032036227093No Hit
CTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCC3530.12410873753638883No Hit
ACTGATGGCTGTCTGAGGCTGGTTGAAGGGGTTAGGGTGATGGCCAGCAG3500.12305398905874247No Hit
GTATGGGAGCACGGAGGTGTATAATTCTGTAGGTCACTCTGCCCTTGCTC3460.12164765775521398No Hit
GTGTGTGTGTGTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTAC3440.12094449210344972No Hit
GCAATGAATCATGGGGCTCTTAGGGCCGGAGCCATGTTCTGAATACCAAG3430.12059290927756762No Hit
GATAAAAAGAGTGATAGAGCGAGGGACTGAGGGAAAGGGAGATGTCATAT3430.12059290927756762No Hit
CATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATTCAAA3410.11988974362580336No Hit
GTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCTTCTTCTCT3410.11988974362580336No Hit
GTGTATGTGTGATTAAGTAAGTGTGTGTGTGTGTGTTTGAGAGTGCTTGT3400.11953816079992126No Hit
CTATTAATCCACCAGTAAACACTTCTTATCACTCTCATTTTCCATCTTTA3330.1170770810187464No Hit
GTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTGCAGTT3330.1170770810187464No Hit
CTATTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCT3330.1170770810187464No Hit
CCAATACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAACA3320.11672549819286428No Hit
GTTCTGTGTCTGGTCTGGAACTATCAGGTATGCTTTGCTTGCTAGCATGA3310.11637391536698215No Hit
CTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCCTAGAGTTC3230.11356125275992518No Hit
GACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTT3230.11356125275992518No Hit
CGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATAC3210.11285808710816093No Hit
CACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCC3210.11285808710816093No Hit
TACATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATTCA3200.11250650428227882No Hit
CATACATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATT3170.11145175580463246No Hit
TCTCTTGACCGGGTGGGAGAACACACCTGTTTCCCCTCTGCCTTTTTTTT3110.10934225884933972No Hit
CCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATACGGGTCCAGA3100.1089906760234576No Hit
CTCTCTCTCTCTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGG3080.10828751037169336No Hit
GAATCATGGGGCTCTTAGGGCCGGAGCCATGTTCTGAATACCAAGCACTT3080.10828751037169336No Hit
GAGCAGGGTGCCATCGCAGTCCTTGCCCCCATTCTTGGCCGCGGGGGCAT3070.10793592754581124No Hit
GGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAACCCAC3060.10758434471992911No Hit
GAGTATCACAAGACCAAATACTGAGTTGCTATCAGTGAATTTGACAGTGA3060.10758434471992911No Hit
CTGCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGC3060.10758434471992911No Hit
AAGCAGGAGGGGGAGGAGATATGACATCTCCCTTTCCCTCAGTCCCTCGC3050.107232761894047No Hit
CTACAGATGGACAGATGGAGAGAATGAGGGATGAAAACTACAGATGGACA3040.10688117906816488No Hit
GTGCGGGACGGAGTGCACCCACTGGCGGAGGCGTGAGTGTAATGCCCCCG3030.10652959624228275No Hit
CATCTACAGTAGTGTTCAGGATTTCATAGTTTCATACATCTGGCCATACT3030.10652959624228275No Hit
GTCCCAGAACAGCAATTGCCCCTTGAACCCACATTAAGTGAGAGTCCATC3020.10617801341640062No Hit
AGGTGGTACACTGAGAGATAAAAAGAGTGATAGAGCGAGGGACTGAGGGA3020.10617801341640062No Hit
CTCTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGGAGTGGAGT3010.10582643059051852No Hit
CCTCAGTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCC2990.10512326493875426No Hit
TCACTGCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAG2970.10442009928699003No Hit
CAGTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCTCTCTCTCTCTCT2960.1040685164611079No Hit
CATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGGAGTGGAGTAAGTGG2960.1040685164611079No Hit
CCCTCAGTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTACCACCTC2930.10301376798346154No Hit
GAGTAAGTGGTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAGGCGTG2920.10266218515757941No Hit
CTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGGAGTGGAGTAA2900.10195901950581518No Hit
TCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTGCCCCC2890.10160743667993305No Hit
GTATATGCATGTATGTGTAGATGACCATATAGAAGTAGGAGTTTGAATAT2890.10160743667993305No Hit
CTTCTGGAGGATCCCAACTGTAAACTGGAGCTACTAGGGTGAGTATCACA2880.10125585385405092No Hit
AAGTAAGTGTGTGTGTGTGTGTTTGAGAGTGCTTGTTTGTATCTTTTTGA2870.10090427102816882No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACC100.007084898145.02269145
CAGTAAT451.0875738E-896.681792
AGCGAAA2700.093.9961851
AGTAATC502.2602762E-887.013613
GGCTACT750.087.013611
ATTAACT1550.074.850415
TAACTGA1550.074.837267
GCGAAAG3400.074.644032
CGAAAGC3500.072.511343
GATGACT406.6556364E-572.49868
ACAATGT300.001934462372.498598
TTAACTG1650.070.3140266
AATTAAC1650.070.3140264
CCATACG2400.069.4900362
TACTGCC955.456968E-1268.6949544
GCCTAAT1950.066.933541
CTAATTG1950.066.933543
CAGTCCG554.0503783E-665.9194145
CGCTGCT2550.065.390897
CCTAATT2000.065.260212
Produced by FastQC (version 0.11.5)