FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840807
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.064.8130951
CGAAAGC2900.050.0169833
ATACGCT450.00962568148.349754
GCGAAAG4100.035.377872
GTATAGG3607.2759576E-1226.1910081
TATAGTA4500.025.7865332
ATAGTAC5400.025.5179253
GTAATAC4109.349606E-1021.226723
GTGTAGG5403.6379788E-1220.1469291
GTACTAT4752.7102942E-1019.8500251
GTATAGT2608.0506325E-519.5270231
GCACTAT2608.0543134E-519.5258626
AGGCTCG2659.3230825E-519.1574485
ACCCGTG2659.348663E-519.1506148
TAGTACC2701.0761025E-418.8026814
ATAATAG3501.9918225E-618.6491893
GGACCGT1950.00682627718.596066
TTAGATA2801.4220345E-418.1311574
TTGTACC6503.6379788E-1217.8522174
GCGTTAG2050.00910427717.6899851
Produced by FastQC (version 0.11.5)