FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC40.2717391304347826No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC40.2717391304347826No Hit
GTATGGAGAACTGAGAAGAACAGGGTCTTTATTAATAGTAATACATAAAA40.2717391304347826No Hit
GGCCAATACAGCGGGTTTGTTAGAACTCTGTTCCAACAAATGAGGGATGT30.20380434782608695No Hit
CTGTATGGAGAACTGAGAAGAACAGGGTCTTTATTAATAGTAATACATAA30.20380434782608695No Hit
GCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCA20.1358695652173913No Hit
GTTAGCAAATGTTGTAAGGAAGATGATGACCAATTCCCAGGACACTGAAA20.1358695652173913No Hit
ATACTAATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTT20.1358695652173913No Hit
CTATCATATTGCTTCAAATCGCTTATATCTTTACAGTCGTCAAAGTCTAC20.1358695652173913No Hit
GCCGTGAGGAGTGCAGGGCGGGGTAGGAGCGGTGGCCACTTCCCTCAGAA20.1358695652173913No Hit
GGCTGTAACATGGTGGAATAGAAATGGACCAGTGACAAGTACTGTTCACT20.1358695652173913No Hit
GCTGCAAAGGGAAGAAGTTTTATTATCTGGGTGGTGTCAAAAGTTCCAAG20.1358695652173913No Hit
TTTCAGACCAGCGCTGGAGCTAGGGTGAGTCCCAATGGCTCTCATTGCCT20.1358695652173913No Hit
GTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATTCCTTTTTGT20.1358695652173913No Hit
GCATAAATGTTATTTGCTCAAAACTACTCTCTGTTATCTTCAATTTATGT20.1358695652173913No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG20.1358695652173913No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA20.1358695652173913No Hit
TCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAA20.1358695652173913No Hit
GCTTAATGCATCCTGTGCAGCAATGGATGATTTCCAACTAATTCCCATGA20.1358695652173913No Hit
ATTATGAGGGACGACTGATCCAGAACAGCTTAACAATAGAGAGAATGGTG20.1358695652173913No Hit
GTTTATATTCTGTGGTATTTGGATGTTAATAAGCCTGAAAGGCCATAGCT20.1358695652173913No Hit
AAGCAGGGACGTGCACAGGAATTTTGAAGGGCAAAACAAAAATCACACAG20.1358695652173913No Hit
GATAAAGGCAGTTAGAGGTGATCTGAATTTCGTTAATAGAGCGAATCAGC20.1358695652173913No Hit
GACCGATTCAATCTGGATAGAGCTCGATGAGATTGGAGAAGATGTAGCTC20.1358695652173913No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAAAACGTT20.1358695652173913No Hit
GGAGACAGACTGTGAAAAGCAAACTGCCTTATAAACCATGTTAAGAATCC20.1358695652173913No Hit
GGTCAATACCTATCAGTGGATCATCAGAAACTGGGAGACTGTTAAAATTC20.1358695652173913No Hit
GGCTCTCTCCCATCCATCGGGCCGAGGCTGATTAGATCTTGAGTGGCTAA20.1358695652173913No Hit
GAAGTGGAGGAAACACTAATCAACAAAGGGCCTCTGCGGGCCAAATCAGC20.1358695652173913No Hit
TAGCTGTAAGGCTTGCATAAATGTTATTTGCTAAAAACTACTCTCTGTTA20.1358695652173913No Hit
GTGTTGGAGTTGATCAGGATGATCAAACGTGGAATCAATGACAGAAACTT20.1358695652173913No Hit
TTGCTGCACAGGATGCATTAAGCAAAGCAGTATTAATGTATACCCCCTTC20.1358695652173913No Hit
CTGCAGGACATTGAGAGTGAGGAGAAGATTCCAAGAACTAAAAACATGAA20.1358695652173913No Hit
GTCTAATATTGCAAATAGGAAATATTGTTTCAATATGGATCAGCCACTCA20.1358695652173913No Hit
GACAGAGACATTGCATTGTGATAAAGATGACAATGTGTAGGCTAATTCAT20.1358695652173913No Hit
CTCCAACACCATTGTTCCAACTCCTTTGACTGCAGCGCCAGCAGCTCCAG20.1358695652173913No Hit
GGGTTAATACACCAGTGTGTTTACCAATAATGAGGACACAGGGACTTTTT20.1358695652173913No Hit
TTCCTATCCAAACACCATTACCATACCTGTATGAAAACCCCTTTACTCCG20.1358695652173913No Hit
CCCTAATGAAGTGGGAGCCAGAATACTAACATCGGAATCGCAATTAACGA20.1358695652173913No Hit
GAAAAAGAGAGAGACAGAGAGAGAGAGACTGACTTGATGCTAATGGCGAA20.1358695652173913No Hit
GCTGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTACTCTCTGTTATC20.1358695652173913No Hit
GGTTAATACACCAGTGTGTTTACCAATAATGAGGACACAGGGACTTTTTC20.1358695652173913No Hit
ACTCTGTACACACCAACAGGACAGCAGAAAAAGACCTGGTCCCTTTGTTC20.1358695652173913No Hit
CGGCAGGTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAACTGAC20.1358695652173913No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGT20.1358695652173913No Hit
ATGATGTTCTTATCCATTATCGCCTGGTCCATTCTGACACAAAGAGGGCC20.1358695652173913No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGCCCC20.1358695652173913No Hit
GTCTCTCACTCTTCTCTTTCTCTGAAAATGAGTTGTGATCTCTACTTCTT20.1358695652173913No Hit
GAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGG20.1358695652173913No Hit
ACATAAAACACTCAAATACAGCAATGTAATGTAATTATAATAAATTGCCA20.1358695652173913No Hit
GTATAGCATTGTAGGGTTCTGAGACCACTGAATTTTAACAGTCTCCCAGT20.1358695652173913No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA20.1358695652173913No Hit
GGTGATGTCTGGGGCCGGGAATGTGCGCAGACTCGCGGCCATCAGTCCCT20.1358695652173913No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTC100.0063819443145.03
GGTCCAT100.0063819443145.06
GTCCATA100.0063819443145.07
TCCATAC100.0063819443145.08
AGGTCCA100.0063819443145.05
CCATACA100.0063819443145.09
CAGGTCC100.0063819443145.04
CGGCAGG100.0063819443145.01
GGCAGGC200.005690396329.020-24
Produced by FastQC (version 0.11.5)