FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563393
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC15740.27937869302600493No Hit
GTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATT11140.19773053623314452No Hit
GTGTAGGCTAATTCATTAATGTGAAACAGGCTCATATTTTCAGACTAATA9100.16152135365544124No Hit
AAGCAGGGACGTGCACAGGAATTTTGAAGGGCAAAACAAAAATCACACAG8980.15939140173910574No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC8670.15388902595523907No Hit
GTGATAAAGATGACAATGTGTAGGCTAATTCATTAATGTGAAACAGGCTC8590.1524690580110154No Hit
GTATGGAGAACTGAGAAGAACAGGGTCTTTATTAATAGTAATACATAAAA7150.12690963501498953No Hit
GACAGAGACATTGCATTGTGATAAAGATGACAATGTGTAGGCTAATTCAT6420.11395242752394864No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6320.11217746759366908No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG6260.11111249163550134No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA6140.10898253971916584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1850.0101.9091641
AACACTG3950.067.916885
TCCCAAC4150.066.3968051
AGGGTAT451.1929306E-464.449656
GATAGCA1700.059.710716
ACACTGT4500.059.615936
TTTAAAC2200.059.3229753
GGGATGA1350.059.0840951
CGAAAGC3350.058.437563
GGATAGC1750.058.004695
TATTACA4100.056.5899352
CAAGGGT2600.055.773744
GGACGTG1301.8189894E-1255.7687877
AGGGTGT2350.055.5364046
GGTATAG4200.055.2376569
TCCGTGG400.006045463554.384221
TAACCAT400.006047587454.379395
TCGGTCC400.006047587454.37939145
GGTCGTT1750.053.8567167
CTGTGTC5250.053.8567169
Produced by FastQC (version 0.11.5)