FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT70.4100761570005858No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT60.35149384885764495No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC60.35149384885764495No Hit
GAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGA40.23432923257176333No Hit
CAATAGGAGAGTGCCCAAAGTATGTCAGGAGTACAAAATTAAGGATGGTT40.23432923257176333No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA30.17574692442882248No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG30.17574692442882248No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAGGCAATGAAAGAGTAT30.17574692442882248No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC30.17574692442882248No Hit
GTTCTTGAGATAGGAGATATGCTTCTAAGAAGTGCAATAGGCCAGGTGTC30.17574692442882248No Hit
CTCTAACATGTATGCAACCATCAAAGGGGAAATTTTGCAATTCTGGAGTT30.17574692442882248No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA30.17574692442882248No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC30.17574692442882248No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA30.17574692442882248No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA20.11716461628588166No Hit
CCGTTAATTTCAATGTTTGCAGATTGCCCGTAAGCACTTCTTCCTCTCTC20.11716461628588166No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT20.11716461628588166No Hit
ATGCTGGCCCATATCTCCTATCTTCTTTGCCCAGAATGAGGAATCCCCTT20.11716461628588166No Hit
AGTTAGAGGTGATCTGAATTTCGTTAATAGAGCGAATCAGCGGTTGAATC20.11716461628588166No Hit
CTGTAGTGCTGGCCAGAACCATTCTGTTCTCATGTCTTATTAATGGATTG20.11716461628588166No Hit
GCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCAGCAGAGGCCATGGA20.11716461628588166No Hit
CACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAA20.11716461628588166No Hit
TCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAA20.11716461628588166No Hit
GCCTCATACAATCCAATCCTGTTAGTTCAGGATGTTGAACGAAGCTTCCG20.11716461628588166No Hit
CCCCCAGGGAGACCAAAAGCACCAGTGAACTGGCGACAGTTGAGTAGATC20.11716461628588166No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG20.11716461628588166No Hit
CATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCA20.11716461628588166No Hit
GTGTAAACGGTTCATGTTTTACCATAATGACCGATGGCCCGAATAATGGA20.11716461628588166No Hit
GTTCAAGAGTACTTGATACCATAGTATCCATGTTTTCATTTGAAGCAATT20.11716461628588166No Hit
CATTGAGAGTGAGGAGAAGATTCCAAGAACTAAAAACATGAAGAAAACGA20.11716461628588166No Hit
GTCAATAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGAT20.11716461628588166No Hit
CCTTAGAACAGCGGATTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG20.11716461628588166No Hit
GTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATT20.11716461628588166No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC20.11716461628588166No Hit
GCCCATGTTCTTGTATGTAAGGACAAATGGAACCTCAAAAATTAAAATGA20.11716461628588166No Hit
GGATAGAGCTCGATGAGATTGGAGAAGATGTAGCTCCAATTGAACACATT20.11716461628588166No Hit
GATTAAAACCAGACTATTCACCATAAGACAAGAAATGGCTAGCAGAGGCC20.11716461628588166No Hit
CTGCAGGACATTGAGAGTGAGGAGAAGATTCCAAGAACTAAAAACATGAA20.11716461628588166No Hit
GAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGG20.11716461628588166No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGTTCCTC20.11716461628588166No Hit
GATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAA20.11716461628588166No Hit
CTGGAGGACCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTT20.11716461628588166No Hit
CTTCATATCCCTCATGCACCTTTAATTTCAATGTTTGCAGATTGCCCGTA20.11716461628588166No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG20.11716461628588166No Hit
GTTGTATGCTGATTTGGCCCGCAGAGGCCCTTTGTTGATTAGTGTTTCCT20.11716461628588166No Hit
GAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGG20.11716461628588166No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.11716461628588166No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT20.11716461628588166No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC20.11716461628588166No Hit
CCTTTAATTTCAATGTTTGCAGATTGCCCGTAAGCACTTCTTCCTCTCTC20.11716461628588166No Hit
CAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGA20.11716461628588166No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA20.11716461628588166No Hit
GAGTAAAGGGGTTTTCATACAGGTATGGTAATGGTGTTTGGATAGGAAGG20.11716461628588166No Hit
CCTCAATGCTTAATTTTAAAGCATCCATCAGCAGGAATTTTGACCGCTGA20.11716461628588166No Hit
GAGCAAATGGCTGGATCGAGTGAGCAGGCAGCAGAGGCCATGGAGGTTGC20.11716461628588166No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20.11716461628588166No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA20.11716461628588166No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGACGATAAAT20.11716461628588166No Hit
GGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGC20.11716461628588166No Hit
GCCCAGTACCTGCTTCTCAGTTCAAGAGTACTTGATACCATAGTATCCAT20.11716461628588166No Hit
GGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAATATGCGCAACC20.11716461628588166No Hit
GAATATATAATGAAGGGGGTATACATTAATACTGCTTTGCTTAATGCATC20.11716461628588166No Hit
CAAGAACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTG20.11716461628588166No Hit
GTGTTCAATTGGAGCTACATCTTCTCCAATCTCATCGAGCTCTATCCAGA20.11716461628588166No Hit
ATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATT20.11716461628588166No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA20.11716461628588166No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20.11716461628588166No Hit
GATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCT20.11716461628588166No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20.11716461628588166No Hit
AGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATAT20.11716461628588166No Hit
ATTGAACACATTGCAAGCATGAGAAGGAATTACTTCACAGCAGAGGTGTC20.11716461628588166No Hit
GTTATAATGTGAAGACACTACAGAGACATAAGCATTTTCTGTATGATAGA20.11716461628588166No Hit
AATGGAACCTCAAAAATTAAAATGAAATGGGGAATGGAGATGAGGCGTTG20.11716461628588166No Hit
GCTAATAACGTCCTGCATACTTTCCCAATGGAACCTTCTTCCACTCCTTT20.11716461628588166No Hit
GTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGA20.11716461628588166No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC20.11716461628588166No Hit
AATGAAGTGGGAGCCAGAATACTAACATCGGAATCGCAATTAACGATAAC20.11716461628588166No Hit
GTATAATAACTGAAACCATAAAAAGTTGGAGGAAGCGAATATTAAGAACA20.11716461628588166No Hit
GTCTGGGGTCAATGAGTCTGCAGACATGAGTATTGGAGTCACTGTCATCA20.11716461628588166No Hit
TCCTTGTTCATTCTGATGATGATAACCATACCATCCATCTATCATTCCAG20.11716461628588166No Hit
TCCATAGCCTTAGCTGTAGTGCTGGCCAGAACCATTCTGTTCTCATGTCT20.11716461628588166No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)