FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901429
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC24480.2715688090798055No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT23540.26114092180304826No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC20380.22608547095777926No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20370.2259745359867499No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA17960.19923920796868083No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC15950.17694127879178506No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC14960.16595871665988116No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC14920.16551497677576382No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT14830.1645165620364998No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA13730.15231371522327328No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG13550.1503168857447453No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG13530.1500950158026866No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC13510.14987314586062797No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA13400.14865286117930532No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC13350.14809818632415864No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC13270.14721070655592397No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT13040.14465920222224934No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT13020.14443733228019068No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC12610.13988899846798805No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA12380.1373374941343134No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA11950.1325672903800521No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG11790.1307923308435828No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA11720.13001578604637748No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG11600.12868456639402548No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC11510.1276861516547615No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC11280.12513464732108684No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA11230.12457997246594019No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA10100.11204432073962564No Hit
GTTGTAAAATGAAATGGGGAATGGAGATGAGGCGTTGCCTCCTCCAATCA10070.11171151582653764No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT9980.11071310108727364No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG9830.10904907652183365No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA9680.1073850519563937No Hit
ATCTCAATGCATGTGTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA9650.10705224704330568No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG9620.10671944213021768No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA9610.10660850715918835No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT9270.10283671814419107No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT9040.1002852138105164No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA9030.10017427883948708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTC100.0070911124144.99758
AGCGAAA256.450826E-6116.049511
ATCCCGG259.3870854E-486.998505145
GTAGAAA5350.062.3630521
CAGGGTG350.003561149862.165925
GGCAGCC350.003566633262.14179145
TCCCAAC2450.059.208931
GATTAAG1350.059.099281
GTACTGA3200.054.3951846
TCGGTCC400.006051105454.374065145
CTACTAG1101.6680133E-952.7497751
TTATATT2100.051.807812
CCCAACA2800.051.807812
TCTCCGC701.6960239E-551.784824145
CTGTGTC2850.050.8763169
GTCAATT1004.686808E-850.771662
TAGATAT2000.050.771663
TACTAGT1152.4738256E-950.4563032
CAAGGGT2600.050.191448
TACTGAT3550.049.013247
Produced by FastQC (version 0.11.5)