FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804900
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT19400.2410237296558579No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18980.23580569014784447No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17730.22027581065970928No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA16610.20636103863834018No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG15190.1887190955398186No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC14920.1853646415703814No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14520.18039508013417818No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14270.17728910423655111No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA13090.16262889799975153No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12760.15852900981488383No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG12440.15455336066592124No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG12010.14921108212200274No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG12000.14908684308609765No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11980.1488383650142875No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11650.14473847682941982No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11450.14225369611131816No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT11250.13976891539321656No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT10750.13355696359796249No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC10650.1323145732389117No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA10580.13144489998757608No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10510.13057522673624053No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA10350.12858740216175923No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT9850.12237545036650516No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT9800.12175425518697976No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT9600.11926947446887812No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA9330.11591502049944093No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG9220.11454839110448503No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9210.11442415206857995No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC9190.11417567399676978No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC9050.11243632749409864No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT8990.11169089327866817No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA8950.11119393713504783No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT8900.11057274195552243No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG8850.10995154677599703No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG8740.10858491738104112No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT8730.10846067834513604No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT8640.10734252702199032No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA8570.10647285377065475No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT8480.10535470244750901No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT8330.1034911169089328No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG8300.10311839980121755No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC8290.10299416076531245No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT8210.1020002484780718No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC8050.1000124239035905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA403.274181E-11126.880281
AGTAGAA2500.087.003621
CAATTCG2100.082.855449
GACAGTA1400.077.676987
TTTAAAC2600.069.714443
GCAGGTA1150.069.350712
CACGAGC451.1938391E-464.443115145
GTAGAAA3450.063.0460972
GGTACTG1300.061.3487055
CAGGTAC1400.056.9666563
GCCCACC902.0492735E-856.38773145
CCTTATA5050.054.5567252
TCGATCG400.006049463554.3772624
TTTTAAA3400.053.3110432
ATATAAT553.2179645E-452.729461
TTATACT5250.052.4783744
CCCTTAT5350.051.497471
GCGAAAG1004.7002686E-850.7521062
ACAATTC3500.049.7132648
CGAAAGC1203.61797E-948.3353423
Produced by FastQC (version 0.11.5)