FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGC11.639344262295082No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.639344262295082No Hit
CAAGAATCCACAATATCACGTGCAAAATCCCAATGATACTTGCGGCAACA11.639344262295082No Hit
ACTCCAACACCATTGTTCCAACTCCTTTGACTGCAGCGCCAGCAGCTCCA11.639344262295082No Hit
GACAGAAAGTGGATGAGAGAACTCATCCTTTATGACAAAGAAGAAATAAG11.639344262295082No Hit
ATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCATGAGGACATGATGCC11.639344262295082No Hit
TCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCC11.639344262295082No Hit
GTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATCAT11.639344262295082No Hit
ACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTG11.639344262295082No Hit
CTTGGAAGTATGCTTCAGATTTTCATTTCATAAATTAGCAATTCGAGTCA11.639344262295082No Hit
GTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATA11.639344262295082No Hit
ATGATGTTCTTATCCATTATCGCCTGGTCCATTCTGACACAAAGAGGGCC11.639344262295082No Hit
ATTTTGGACAAAGCGTCTACGCTGCAGTCCCCGCTCACTGGGCACGGTGA11.639344262295082No Hit
CTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCAT11.639344262295082No Hit
GGCGGGGATGTTCTTCCAGATATTTATTCATCCTCTCGTCAAAAGCAGAG11.639344262295082No Hit
GACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATACTCTTTG11.639344262295082No Hit
AGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGA11.639344262295082No Hit
CCCCAGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCCGAACG11.639344262295082No Hit
TGGGCTGTGTGTGGGGTGTGCGCGGTGTTCTGCCCGTGTTGGCCCCAAGC11.639344262295082No Hit
CTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCT11.639344262295082No Hit
AAATAGCACTCAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTC11.639344262295082No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA11.639344262295082No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT11.639344262295082No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA11.639344262295082No Hit
CTGGGGTTGGAGTTGATCAGGATGATCACACGTGGAATCAATGACAGAAA11.639344262295082No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC11.639344262295082No Hit
TCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTT11.639344262295082No Hit
AGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTG11.639344262295082No Hit
CTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGG11.639344262295082No Hit
GGGAGAAGACCATGGACACAATATTTACTCCGAAAGTGACGGCTCGTCGA11.639344262295082No Hit
TCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAA11.639344262295082No Hit
GAAATACACCAAGAAAATATACTGGTGGGATGGGCTCCAATCATCCGACG11.639344262295082No Hit
CCCCTTGGAAGTACTCTTGTCCCTCTGATGAAGCTTGATACTCTTAGATC11.639344262295082No Hit
GTCTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTACTTGATCCA11.639344262295082No Hit
AAGTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAAAA11.639344262295082No Hit
ATACGCCGCAGCATTGTCGCCGAAGAAATACCACACTTCATTTCTGATGT11.639344262295082No Hit
GTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTG11.639344262295082No Hit
GACCCGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACC11.639344262295082No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA11.639344262295082No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT11.639344262295082No Hit
TTGTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT11.639344262295082No Hit
TTAATTGGCTTTTTACTTTCTCATACAGATTCTTCACATTTGAGTCATGA11.639344262295082No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG11.639344262295082No Hit
CACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGA11.639344262295082No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA11.639344262295082No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT11.639344262295082No Hit
TGTAAGAGGTAATTCTCCAGTATTCAATTACAACAATACCACTAACAGAC11.639344262295082No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA11.639344262295082No Hit
GCCACTTTCTGCTTGGGCATGAGCATGAACCAGTCCCTTGACATTTCTTC11.639344262295082No Hit
GTGGATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCC11.639344262295082No Hit
GTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACGACAATTGGT11.639344262295082No Hit
GTCTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAA11.639344262295082No Hit
ACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCTCA11.639344262295082No Hit
TACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCC11.639344262295082No Hit
TCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATA11.639344262295082No Hit
CACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCAC11.639344262295082No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC11.639344262295082No Hit
CTCCACATTCCTGAAGTCTGCTTGAAATGGGAATTGATGGATGAGGATTA11.639344262295082No Hit
GAGCACCAAGGCTTGTTGTGGCGACCTGCGAGGTGTCACTTGTGCCTTGC11.639344262295082No Hit
ATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGCACAAAGAGCAAA11.639344262295082No Hit
ACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACA11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT50.0145.06
TAGCCCC50.0145.0145
ACTTTTT50.0145.08
AAGGTAC50.0145.03
CAAGGTA50.0145.02
ACAAGGT50.0145.01
AGGTACT50.0145.04
TACTTTT50.0145.07
GGTACTT50.0145.05
CTTTTTT50.0145.09
Produced by FastQC (version 0.11.5)