FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGAGCTGGGGAATTGAATCCAGCGACAATGTGATGGGAATGATCGG13.4482758620689653No Hit
TCCCACGAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTTT13.4482758620689653No Hit
GCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTAGCAAATGCTCCTAG13.4482758620689653No Hit
CAGTTGATCGAGAACATTTACGCTTTGTGAACAGTAGTGAATTAATTTTT13.4482758620689653No Hit
GGAAAAATACTGTGTTCTTGAAATAGTAGACATGCTCTTGATGACTGAGA13.4482758620689653No Hit
CAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCT13.4482758620689653No Hit
CGTCGGGAGACAATAGTACGTTCCCTCTTTGATCTCTCACCCTTAAAAAT13.4482758620689653No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA13.4482758620689653No Hit
GTCAACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGG13.4482758620689653No Hit
TAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGA13.4482758620689653No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGACGAAACCACCACAAGTTGT13.4482758620689653No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTCCATTAG13.4482758620689653No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAGTCCGGGCCCT13.4482758620689653No Hit
GATAAAAGAGGTAACGTACTATTGTCTCCCGAAGAAGTCAGAGAAACGCA13.4482758620689653No Hit
CAATAACTTATTCGTCAACAATGATGAGGGAGATCAATTGCCATGAGTCA13.4482758620689653No Hit
GGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC13.4482758620689653No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTT13.4482758620689653No Hit
GGAAGGGTTCGAGCCGAACGGCTGCATTGAGGCCAAGCTATCACAACTCA13.4482758620689653No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGATTCAATCC13.4482758620689653No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGAATTTATGCTGGTATTTGT13.4482758620689653No Hit
CTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACAGTGTTACAATA13.4482758620689653No Hit
TCACTGAGTCCTGGGATGATGATGGGCATGTTAATCATGCTAAGTACGGT13.4482758620689653No Hit
GTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCT13.4482758620689653No Hit
GTAATGGAATCAATGCAAAAAGTGGAAATAGAGATCACAACCCAATTTCA13.4482758620689653No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA13.4482758620689653No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT13.4482758620689653No Hit
GGCTAAAGACAACACCGATCCTGTCACCTCTGACTAAGGGGATTTTAGGA13.4482758620689653No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTAGAAAGGTTGAAACATG13.4482758620689653No Hit
AGCCAGCATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCAGCAAT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)