FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853946
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11290.1322097650202706No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10830.12682300754380255No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9740.11405873439304126No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG752.547146E-548.343355
AGCGAAA2000.043.5090141
CGAAAGC3000.031.4231783
ATATACT9500.026.716064
TAGCGGG1409.981313E-425.8982244
GCGAAAG3657.2759576E-1225.8272672
TATATAC16500.025.490133
TATACAG7400.025.478255
GTATATA13450.024.8006761
ATACTTA9500.024.4261136
GTTATAT3854.2564352E-1022.6020871
CATATAT10400.022.3123151
TATACTT10400.021.6150555
GTTACGC2053.6665064E-421.2189378
TACGCAG3106.2293475E-721.0527485
CAGCTCG7250.020.9994329
TAATAGG2104.3073954E-420.7185764
TACTTAC10050.020.1984587
CTACACT3601.1729753E-720.1430634
ATATATA11200.020.0711232
Produced by FastQC (version 0.11.5)