FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871060
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11530.13236746033568295No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10990.1261681170068652No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9840.1129658117695681No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.052.456761
TATACCG450.00963150348.34255
TGTAGCG850.002739243734.124122
GTATTAG2755.456968E-1231.6423631
CGAAAGC3950.031.2084523
GCGAAAG4100.030.0666772
TATACAG9100.029.4836125
CGTGCTA1306.48379E-427.88358
ATATACT9150.026.1524984
TATATAC17650.025.4722523
CAGCTCG9250.025.080089
TATACGT1500.00149246924.1712514
TATAGTG3902.0008883E-1124.171255
TATACTT9850.023.5577665
TTATAGT4154.3655746E-1122.715154
TAGTACT3203.3774995E-822.6605474
GTATATA14850.022.4621731
ATACTTA9550.022.0198826
TATAATA5400.021.4855582
ATACAGC11650.021.1628116
Produced by FastQC (version 0.11.5)