FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA90.6160164271047228No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT90.6160164271047228No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA80.5475701574264202No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC70.4791238877481177No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT70.4791238877481177No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60.41067761806981523No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG60.41067761806981523No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG60.41067761806981523No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA50.34223134839151265No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.34223134839151265No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT50.34223134839151265No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA40.2737850787132101No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.2737850787132101No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA40.2737850787132101No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT40.2737850787132101No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.2737850787132101No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC40.2737850787132101No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.2737850787132101No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.2737850787132101No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA40.2737850787132101No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.20533880903490762No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA30.20533880903490762No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT30.20533880903490762No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.20533880903490762No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.20533880903490762No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA30.20533880903490762No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT30.20533880903490762No Hit
AAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTT30.20533880903490762No Hit
GATGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA30.20533880903490762No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC30.20533880903490762No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.20533880903490762No Hit
ATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGG30.20533880903490762No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.20533880903490762No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAA30.20533880903490762No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.20533880903490762No Hit
CCATAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAA30.20533880903490762No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.20533880903490762No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30.20533880903490762No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.20533880903490762No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA30.20533880903490762No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.20533880903490762No Hit
AATATATTCAATATGGAGAGAATAAAAGCACTGAGAGATCTAATGTCGCA20.13689253935660506No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA20.13689253935660506No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAG20.13689253935660506No Hit
GAATAATGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCA20.13689253935660506No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.13689253935660506No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC20.13689253935660506No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.13689253935660506No Hit
CTTCCTTCAATTATCTCAAATCGGTGCTTCAATAGTGCATTCGGGTCACC20.13689253935660506No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.13689253935660506No Hit
GCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTT20.13689253935660506No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.13689253935660506No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA20.13689253935660506No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGCCACTAATTGATGGCCATCC20.13689253935660506No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTTGTCG20.13689253935660506No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT20.13689253935660506No Hit
GATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCACACATTTGGA20.13689253935660506No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA20.13689253935660506No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT20.13689253935660506No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.13689253935660506No Hit
CTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACG20.13689253935660506No Hit
GGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAG20.13689253935660506No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACATCAGGA20.13689253935660506No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC20.13689253935660506No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC20.13689253935660506No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC20.13689253935660506No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20.13689253935660506No Hit
CAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCT20.13689253935660506No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT20.13689253935660506No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.13689253935660506No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA20.13689253935660506No Hit
GCGGGAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.13689253935660506No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.13689253935660506No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG20.13689253935660506No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA20.13689253935660506No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA20.13689253935660506No Hit
GGCTTGTGTTGAGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTC20.13689253935660506No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA20.13689253935660506No Hit
TGCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTA20.13689253935660506No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG20.13689253935660506No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.13689253935660506No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20.13689253935660506No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC20.13689253935660506No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA20.13689253935660506No Hit
TATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTC20.13689253935660506No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT20.13689253935660506No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA20.13689253935660506No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA20.13689253935660506No Hit
GAGAATAATGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTG20.13689253935660506No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT20.13689253935660506No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.13689253935660506No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG20.13689253935660506No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT20.13689253935660506No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC20.13689253935660506No Hit
GTACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATG20.13689253935660506No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20.13689253935660506No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT20.13689253935660506No Hit
ATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGC20.13689253935660506No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.13689253935660506No Hit
GAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAA20.13689253935660506No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGCTCTAATG20.13689253935660506No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20.13689253935660506No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC20.13689253935660506No Hit
CAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTT20.13689253935660506No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG20.13689253935660506No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA20.13689253935660506No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC20.13689253935660506No Hit
GGGCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.13689253935660506No Hit
CCAATACTCAGAAAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCG20.13689253935660506No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20.13689253935660506No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT20.13689253935660506No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC20.13689253935660506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG400.007201893718.125140-144
Produced by FastQC (version 0.11.5)