FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807017
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA82851.0266202570701732No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG67290.8338114314816168No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA57100.7075439550839697No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT54140.6708656694964295No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA49130.6087851928769779No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC38400.47582640762214423No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT38120.47235684006656614No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT37930.47000249065385236No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC37860.4691350987649579No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG37730.4675242281141538No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG37490.46455031306651534No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG32890.4075502746534459No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG32180.39875244263751564No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC31790.3939198306851033No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG30590.3790502554469113No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA30230.3745893828754537No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30140.37347416473258926No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT29610.36690676900238783No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG28270.3503024099864067No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC27930.346089363668919No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT27100.33580457412916953No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG26390.32700674211323927No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG26000.32217413016082685No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC24180.29962194104956896No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG24130.2990023754146443No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC23900.2961523734939908No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG23080.2859914970812263No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG22930.28413280017645226No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20180.2500566902555956No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA19640.2433653813984092No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC19580.2426219026364996No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG19510.24175451074760507No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA18690.23159363433484054No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAAGC18470.228867545541172No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA18240.22601754362051854No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG18140.2247784123506692No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG17300.21436970968393476No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA17120.21213927339820599No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA16660.20643926955689904No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16580.2054479645410196No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC16300.20197839698544146No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA15810.1959066537631797No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG15710.19466752249333036No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT15700.19454360936634543No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT15470.19169360744569197No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA15150.18772838738217412No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA14770.18301968855674663No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA14750.18277186230277678No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14490.17955012100116852No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG14100.1747175090487561No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT14050.17409794341383145No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA13970.17310663839795196No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA13920.1724870727630273No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT13840.17149576774714784No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA13530.16765446081061489No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC13100.16232619635026274No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC13030.16145880446136823No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA12980.16083923882644355No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG12910.15997184693754904No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT12890.15972402068357916No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAAGCTGGAATCAACAAGGAT12830.15898054192166955No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC12770.15823706315975997No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA12740.15786532377880516No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT12730.15774141065182024No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT12680.15712184501689555No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC12600.1561305400010161No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC12090.14981097052478448No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC11920.1477044473660406No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11860.14696096860413102No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11750.14559792420729675No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA11670.1446066191914173No Hit
GAAAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCTC11540.14299574854061314No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11450.14188053039774876No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11260.139526180985035No Hit
TCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT11020.1365522659373966No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC10980.13605661342945688No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA10400.12886965206433074No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA10270.12725878141352662No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG10070.12478051887382793No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC9950.12329356135000874No Hit
GTACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATG9840.12193051695317447No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA9840.12193051695317447No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG9830.12180660382618955No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA9780.12118703819126488No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC9770.12106312506427992No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT9740.12069138568332513No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA9710.12031964630237033No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG9660.11970008066744568No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT9450.11709790500076206No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA9440.11697399187377713No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC9430.11685007874679221No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA9100.11276094555628939No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG9090.11263703242930447No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG8900.11028268301659072No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA8840.10953920425468112No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG8790.10891963861975645No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA8510.10545007106417834No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT8450.10470659230226871No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC8420.10433485292131392No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC8390.10396311354035911No Hit
CCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTG8260.102352242889555No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC8240.10210441663558512No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC8230.10198050350860019No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC8220.10185659038161525No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC8180.10136093787367552No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT8170.1012370247466906No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG8170.1012370247466906No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA8140.1008652853657358No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT8120.10061745911176592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA10500.0117.41211
AGCGAAA1550.0116.966521
GCAGGTA5950.0114.5682142
AGCGAGC203.8645611E-4108.758644145
CAGGTAC6350.0107.351323
GGTACTG6600.0102.18625
CCGCACG406.113496E-790.6322145
GAGCGAA259.376195E-487.023091
GGCGAAA353.4323515E-582.8791351
GCGAAAG2550.082.472862
ACTGATC8800.077.439748
AGGTACT9550.073.6582954
CGAAAGC2500.072.519253
TATAGAG300.001933569672.519242
CACGAGC502.3047469E-672.50577145
GTAGAAA17250.072.3090442
GGTCAAA7250.070.018581
TAGAAAC18800.067.118873
GTCAAAT7700.066.868392
GACAGTA7550.065.295117
Produced by FastQC (version 0.11.5)