FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATACAATATGGAGAGAATAAAAGAACTGAGAGATCAAATGTAGCA13.4482758620689653No Hit
GGGAAGATCCGAAAATCGAAACTAACCAGTATGCTGCAATATGCACACAT13.4482758620689653No Hit
GTAAATTCTGTTATTGAAAAGATGAATACACAGTTCACAGCAGTAGGTAA13.4482758620689653No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAATGCAAGA13.4482758620689653No Hit
GTATGCTAGAGTCCCGGTTTCGTTTCATTAACAACACTAAGTCCCCTTGC13.4482758620689653No Hit
ATAGAAGTGTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCT13.4482758620689653No Hit
GTACAAATTATTTAAAAATCACATAATATATTCATTATAAATGTAATATA13.4482758620689653No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA13.4482758620689653No Hit
CAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATT13.4482758620689653No Hit
CCCATCATAATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGG13.4482758620689653No Hit
TCGCAGTCACCAACTCTCGAGATACACACTAACTCGCCTGTGTACCAGAT13.4482758620689653No Hit
AATCAAACCGGTATTATATATTTAATGAATCTGAAAATATAATGGAATTT13.4482758620689653No Hit
CTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCT13.4482758620689653No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT13.4482758620689653No Hit
GGGACTGCTGGATTGCTTCATAAAGCCCCGGAAGAAGGATGGTACGAGCT13.4482758620689653No Hit
TAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGG13.4482758620689653No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGACAATCGAAACTAA13.4482758620689653No Hit
GAGCATGACTCCTATTTCAAGAACACAGTATATTTCCCATTTGTGTGGCT13.4482758620689653No Hit
CAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAATGT13.4482758620689653No Hit
CTCTAGGTAATATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATGA13.4482758620689653No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA13.4482758620689653No Hit
GTCTAAGGATGTCAACCATCCTTACGCCTCCAATCTGTGTGCTGTGGCCC13.4482758620689653No Hit
GATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTTC13.4482758620689653No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13.4482758620689653No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA13.4482758620689653No Hit
CCTTTCGTCAGTCCGAAAGAGGCGATGAGACAATTGAAGAAAAATTTGAG13.4482758620689653No Hit
ATGCTACTATTTGCTATCCATACTGTCCAAAAAAGTACCTTGTTTCTACC13.4482758620689653No Hit
GCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTT13.4482758620689653No Hit
ACGCGAATCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCAT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)