FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA22.8169014084507045No Hit
GGGCCAATATAGTCAATATGGAGAGAATAAAAGACCGGAGAGCTCACGGT11.4084507042253522No Hit
CCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAAC11.4084507042253522No Hit
CCCAAATGTCAAAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACG11.4084507042253522No Hit
CTTCTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCA11.4084507042253522No Hit
CCGTAGTCGAGACACAACTGACACATGGAAAGACAAAGAACTACTCACAG11.4084507042253522No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.4084507042253522No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACACCTCA11.4084507042253522No Hit
CAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGGCA11.4084507042253522No Hit
AGGGGAGCCACACACATTCAGACGAGAAGATCATTTGAGTTAAAGCAGCT11.4084507042253522No Hit
TCAGAGAACTCTTCTTTCTTGCTCCGTCCAGACTCGAAGTCGACCCTGGC11.4084507042253522No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT11.4084507042253522No Hit
CAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTA11.4084507042253522No Hit
ATCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCAC11.4084507042253522No Hit
CAATGTAGCCTTTGCAGATTGGCTTGTTCCACTTATTAATATGTATTGAA11.4084507042253522No Hit
GGCCAGGCAAGGAAAGAATATGGGGAAGATCCGCAAATCGAAACTCACAA11.4084507042253522No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA11.4084507042253522No Hit
CTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGC11.4084507042253522No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATA11.4084507042253522No Hit
TTCTATACCTAGTAACCATATTTTTAGTCAAATAAATGAGCCTTGCGTGG11.4084507042253522No Hit
TCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAAC11.4084507042253522No Hit
GGGATGGATGGAGAAATGAGCAATGGGAAACTGGTGGGACACCCTGAGCA11.4084507042253522No Hit
AAACAAGGCATTTTTTCATGAAGGAGAAGTTTAATTCTTTATTTTTGCAG11.4084507042253522No Hit
GAATAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTG11.4084507042253522No Hit
ACCGTACCCATAAACCAACCCTCCTCATACAAGGTAAAGGTGACTAACGT11.4084507042253522No Hit
ATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTT11.4084507042253522No Hit
GAGATAGAAAACACAACCAAACGCAGATACAAAACACACTGAGCAACAGA11.4084507042253522No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC11.4084507042253522No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11.4084507042253522No Hit
GCCTTCTCTCCTTTTACAAGATTGCTCAGTTCATTGATGCTTAGCAGACT11.4084507042253522No Hit
AAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGG11.4084507042253522No Hit
CTCTTGATAGTGTCTGTTATTATGCCGTTGTACTTTAACACAGCCACTGC11.4084507042253522No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG11.4084507042253522No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA11.4084507042253522No Hit
GAATCAATAATTGTAGCATCGTGTGTGAATATAGAAAATTTCTTATCATT11.4084507042253522No Hit
GGATTCCCATTTCATCGACGAACGGGTTGAATCAATAATTGTATACTATG11.4084507042253522No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT11.4084507042253522No Hit
CATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTT11.4084507042253522No Hit
GGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTG11.4084507042253522No Hit
CCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTAT11.4084507042253522No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG11.4084507042253522No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCACTATGGAGC11.4084507042253522No Hit
CCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTC11.4084507042253522No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG11.4084507042253522No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT11.4084507042253522No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA11.4084507042253522No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA11.4084507042253522No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG11.4084507042253522No Hit
GAAAGTCATCCATGGCTGCACAGGAAGCATTGAGCAAGGCCGTATTTTTG11.4084507042253522No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCAATCACACCAGTA11.4084507042253522No Hit
CAATCATTGGGGGGAACGAGCCTAACATACTAATACCACATTAGCAAGCA11.4084507042253522No Hit
GATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGGACAAGCG11.4084507042253522No Hit
GTCCTCGCTCACTGGGCACGGGGAGCGTGAACACAAATCCTAAAATTCCC11.4084507042253522No Hit
ATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCATGAGGACATGCTGCC11.4084507042253522No Hit
GACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGAC11.4084507042253522No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC11.4084507042253522No Hit
GAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAG11.4084507042253522No Hit
ACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACT11.4084507042253522No Hit
AAGGCATTCGTGGCAACCAAATAGGCAACATTGACAAGATGAGACTCCCC11.4084507042253522No Hit
ACTGCAGTGCATGTGTGAGGAATGAGGTGAACCAAGATGCATTAAGCAAA11.4084507042253522No Hit
CTGTTCACTCGATACAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAG11.4084507042253522No Hit
GGAATAAACATAACCAAAGAATTAAAAAATCTATACACTGAAAACTAGAA11.4084507042253522No Hit
GAGACGAACCCCGCACTCAGAATGAAGTGGATGGCAATGAGATACCCAAT11.4084507042253522No Hit
ATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGAC11.4084507042253522No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.4084507042253522No Hit
ATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGGACAACCTGGAAC11.4084507042253522No Hit
TTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGC11.4084507042253522No Hit
TCCATTGGTTCTCACATATAGGAACATGGGCCTCGACACTTGGCCTATCG11.4084507042253522No Hit
ATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT11.4084507042253522No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTT50.0145.05
AAATTTT50.0145.06
AATTTTT50.0145.07
TTTTTGT50.0145.09
ATTAAAT50.0145.03
TATTAAA50.0145.02
ATTTTTG50.0145.08
ATATTAA50.0145.01
TTAGCTC50.0145.0145
TTAAATT50.0145.04
Produced by FastQC (version 0.11.5)