FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG14.0No Hit
GCGTCAAATGTCCCAAGCACATAACGCATTTGCTCGAACAGTGTCCTTAA14.0No Hit
AAATAAAGCATGTCAATTGAATGATTGAAGCTGTAAATAAATTGAATAAA14.0No Hit
ACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATC14.0No Hit
CCAGCTTACTGTCTCCTCTGGGTGTAATCGAACTGAGTAGGATTACGCAC14.0No Hit
TACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGAT14.0No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT14.0No Hit
CCTCTTGGGAATCCAGGAATGTGTACTTGGAACGGCATCATATTCCATGC14.0No Hit
CAAGAGTCAATTGGTGTGGATGGCATGCCACTCTGCTGCATTTGAAGATT14.0No Hit
ATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCC14.0No Hit
ATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTG14.0No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT14.0No Hit
GAGTCTGCCGGCCCTGCAAGTTAGTGGGATTCAAAATGAGCAAAAAGAAG14.0No Hit
GCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAA14.0No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC14.0No Hit
GGCAATTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGCCTGA14.0No Hit
AAATACGGCAATGGTGTTTGGATAGGGAGAACTAAAATCATTAGTTCAAG14.0No Hit
AGTCTCTTGTCCCTAAGGCAAACAGAAGCCGGTACAGTGGATTCGTAAGG14.0No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT14.0No Hit
TGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCC14.0No Hit
GCAGATGCATATGTTTTTGTGGGGTCATCAAGATACAGCAAGAAGTTCAA14.0No Hit
GTTAATACTCAAGCATTACCCTTTTTCGTTTGTTGAGAAGAGAACTATGC14.0No Hit
CCCTTTCACTAGTCTTGCCATTTTGTTGGATAACATTATGTGGGCCATTC14.0No Hit
ATGTAATCATCGCCAGCAACATTCGACAATGATGATTTTCCTTCCACATA14.0No Hit
TCTGAGAATAGCTGTTGATCTTCTCCCAACCATTGTGAATTCTGAATACC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)