FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTC40.39370078740157477No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA40.39370078740157477No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT40.39370078740157477No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT40.39370078740157477No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.39370078740157477No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.2952755905511811No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT30.2952755905511811No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.2952755905511811No Hit
GAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACA30.2952755905511811No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT30.2952755905511811No Hit
TCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACA30.2952755905511811No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.2952755905511811No Hit
AACAAAGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAAT30.2952755905511811No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.2952755905511811No Hit
CCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTC30.2952755905511811No Hit
ATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTA30.2952755905511811No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA30.2952755905511811No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG30.2952755905511811No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT30.2952755905511811No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG30.2952755905511811No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT30.2952755905511811No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC30.2952755905511811No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG20.19685039370078738No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAG20.19685039370078738No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG20.19685039370078738No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT20.19685039370078738No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA20.19685039370078738No Hit
CATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAAT20.19685039370078738No Hit
GAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCC20.19685039370078738No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.19685039370078738No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA20.19685039370078738No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG20.19685039370078738No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCC20.19685039370078738No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20.19685039370078738No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.19685039370078738No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC20.19685039370078738No Hit
AGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACCG20.19685039370078738No Hit
ATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTC20.19685039370078738No Hit
ATCCCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGT20.19685039370078738No Hit
GACTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGAC20.19685039370078738No Hit
GCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTT20.19685039370078738No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT20.19685039370078738No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.19685039370078738No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG20.19685039370078738No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.19685039370078738No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA20.19685039370078738No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA20.19685039370078738No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG20.19685039370078738No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA20.19685039370078738No Hit
GTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGG20.19685039370078738No Hit
TCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCA20.19685039370078738No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG20.19685039370078738No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC20.19685039370078738No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.19685039370078738No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA20.19685039370078738No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.19685039370078738No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.19685039370078738No Hit
TCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATT20.19685039370078738No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA20.19685039370078738No Hit
GTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCA20.19685039370078738No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG20.19685039370078738No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC20.19685039370078738No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA20.19685039370078738No Hit
ACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAA20.19685039370078738No Hit
ATATGATGCAATCAAATGCATGATGACATTCTTTGGCTGGAAAGAGCCTA20.19685039370078738No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20.19685039370078738No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.19685039370078738No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.19685039370078738No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.19685039370078738No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA20.19685039370078738No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.19685039370078738No Hit
TTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGT20.19685039370078738No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA20.19685039370078738No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.19685039370078738No Hit
CTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCATGA20.19685039370078738No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC20.19685039370078738No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC20.19685039370078738No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.19685039370078738No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGCCTC20.19685039370078738No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.19685039370078738No Hit
GCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGTAAGCGG20.19685039370078738No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG20.19685039370078738No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20.19685039370078738No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG20.19685039370078738No Hit
ACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATC20.19685039370078738No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.19685039370078738No Hit
GGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGC20.19685039370078738No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.19685039370078738No Hit
GAACAAAGAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAG20.19685039370078738No Hit
CCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAAT20.19685039370078738No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA20.19685039370078738No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT20.19685039370078738No Hit
TGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTA20.19685039370078738No Hit
GGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGA20.19685039370078738No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.19685039370078738No Hit
CTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGC20.19685039370078738No Hit
ATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAACAAG20.19685039370078738No Hit
CTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTC20.19685039370078738No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT20.19685039370078738No Hit
CAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCC20.19685039370078738No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT20.19685039370078738No Hit
CATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGACT20.19685039370078738No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA20.19685039370078738No Hit
GCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAG20.19685039370078738No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA20.19685039370078738No Hit
AGTTTCAACAAAGTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGA20.19685039370078738No Hit
CCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAACAATGC20.19685039370078738No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGAC20.19685039370078738No Hit
GTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGA20.19685039370078738No Hit
GCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATG20.19685039370078738No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT20.19685039370078738No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA20.19685039370078738No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA20.19685039370078738No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.19685039370078738No Hit
GGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGAT20.19685039370078738No Hit
ATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTT20.19685039370078738No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA20.19685039370078738No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC20.19685039370078738No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG20.19685039370078738No Hit
CTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGT20.19685039370078738No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA20.19685039370078738No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG20.19685039370078738No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT20.19685039370078738No Hit
GGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAA20.19685039370078738No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT20.19685039370078738No Hit
ACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCTATATAAA20.19685039370078738No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.19685039370078738No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.19685039370078738No Hit
GAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAG20.19685039370078738No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG20.19685039370078738No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG20.19685039370078738No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG20.19685039370078738No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)