FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA70.3558718861209964No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.3050330452465684No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG60.3050330452465684No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT60.3050330452465684No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC50.2541942043721403No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.2541942043721403No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG50.2541942043721403No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.20335536349771224No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.20335536349771224No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT40.20335536349771224No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA40.20335536349771224No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30.1525165226232842No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATT30.1525165226232842No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG30.1525165226232842No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA30.1525165226232842No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA30.1525165226232842No Hit
GACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGG30.1525165226232842No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.1525165226232842No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC30.1525165226232842No Hit
CCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACT30.1525165226232842No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA30.1525165226232842No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAAC30.1525165226232842No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30.1525165226232842No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.1525165226232842No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA30.1525165226232842No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC30.1525165226232842No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.1525165226232842No Hit
CTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGG30.1525165226232842No Hit
ATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAAT30.1525165226232842No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1525165226232842No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.1525165226232842No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA30.1525165226232842No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGA30.1525165226232842No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.1525165226232842No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.1525165226232842No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.1525165226232842No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.1525165226232842No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.1525165226232842No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.1525165226232842No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.1525165226232842No Hit
GGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCT20.10167768174885612No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG20.10167768174885612No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA20.10167768174885612No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA20.10167768174885612No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.10167768174885612No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.10167768174885612No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA20.10167768174885612No Hit
CTCCTATTTCAAGAACACAGTATTTTTCCCATTTGTGTGGCTCCAGTCTC20.10167768174885612No Hit
ACCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTC20.10167768174885612No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC20.10167768174885612No Hit
TTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCG20.10167768174885612No Hit
CTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAA20.10167768174885612No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC20.10167768174885612No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.10167768174885612No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC20.10167768174885612No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC20.10167768174885612No Hit
CTTCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATC20.10167768174885612No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC20.10167768174885612No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT20.10167768174885612No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT20.10167768174885612No Hit
GTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCT20.10167768174885612No Hit
GATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACA20.10167768174885612No Hit
GGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATCGCTGC20.10167768174885612No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC20.10167768174885612No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.10167768174885612No Hit
GACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGT20.10167768174885612No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC20.10167768174885612No Hit
GAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTTCAAAGAAA20.10167768174885612No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT20.10167768174885612No Hit
TTTTCAAGAAGGTCATCTTTCAGACCAGCACTGGAGCTAGGATGAGTCCC20.10167768174885612No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA20.10167768174885612No Hit
GGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA20.10167768174885612No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA20.10167768174885612No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.10167768174885612No Hit
AGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGA20.10167768174885612No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.10167768174885612No Hit
TTCCAAGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCC20.10167768174885612No Hit
CTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTGTTCCCGCTGAATGCT20.10167768174885612No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20.10167768174885612No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20.10167768174885612No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA20.10167768174885612No Hit
CTCCTCTCCAGTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTA20.10167768174885612No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT20.10167768174885612No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC20.10167768174885612No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT20.10167768174885612No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT20.10167768174885612No Hit
CCCTTTCCCTAGTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGC20.10167768174885612No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC20.10167768174885612No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT20.10167768174885612No Hit
AATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATT20.10167768174885612No Hit
GAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAACAGGCAG20.10167768174885612No Hit
ACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCC20.10167768174885612No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC20.10167768174885612No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.10167768174885612No Hit
CTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAA20.10167768174885612No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG20.10167768174885612No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC20.10167768174885612No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC20.10167768174885612No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA20.10167768174885612No Hit
GCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGT20.10167768174885612No Hit
GATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCT20.10167768174885612No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.10167768174885612No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.10167768174885612No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20.10167768174885612No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG20.10167768174885612No Hit
ATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGT20.10167768174885612No Hit
CACAAATACCAGCAGAAATGCTAGCAAGCATTGACCTGAAGTACTTCAAT20.10167768174885612No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.10167768174885612No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG20.10167768174885612No Hit
GCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATT20.10167768174885612No Hit
GGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAAT20.10167768174885612No Hit
GTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAA20.10167768174885612No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG20.10167768174885612No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA20.10167768174885612No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC20.10167768174885612No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC20.10167768174885612No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.10167768174885612No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.10167768174885612No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC20.10167768174885612No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG20.10167768174885612No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT20.10167768174885612No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAA20.10167768174885612No Hit
ATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAAT20.10167768174885612No Hit
CAGGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAA20.10167768174885612No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT20.10167768174885612No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT20.10167768174885612No Hit
TTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTAAGCTGAT20.10167768174885612No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.10167768174885612No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.10167768174885612No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20.10167768174885612No Hit
ACTTTAGAGCCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGC20.10167768174885612No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.10167768174885612No Hit
AATTGAACCATTCTTGAGGACGACACCACGCCCCCTCAGATTGCCTGATG20.10167768174885612No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20.10167768174885612No Hit
CCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTG20.10167768174885612No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA20.10167768174885612No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCA20.10167768174885612No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA20.10167768174885612No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA20.10167768174885612No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA20.10167768174885612No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG20.10167768174885612No Hit
TGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCC20.10167768174885612No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAATAGGAA20.10167768174885612No Hit
ACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAAT20.10167768174885612No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)