FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717165
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG32090.4474563036400271No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA27150.37857396833364704No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA24290.33869472157732183No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA24210.3375792181715505No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21140.2947717749750755No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20070.27985191692288386No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT19730.2751110274483557No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC18350.2558685936988001No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG18030.2514065800757148No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG17550.24471355964108676No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG16510.23021201536605942No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC15540.21668653657108197No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG14730.2053920645876472No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC14640.20413712325615443No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG14380.2005117371873976No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT14300.19939623378162624No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14050.19591028563859084No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13770.19200602371839118No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT13210.1841974998779918No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG13210.1841974998779918No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG11270.15714654228803693No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG11070.15435778377360856No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10870.15156902525918023No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA10120.14111118083007398No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG9980.13915904986997413No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT9920.13832242231564565No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC9650.13455759832116737No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC9590.13372097076683886No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC9520.13274490528678895No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA9480.1321871535839033No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA8910.12423919181778252No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG8910.12423919181778252No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG8560.11935886441753292No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC8460.11796448516031875No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA8390.11698841968026885No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC8290.11559404042305467No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA8250.115036288720169No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG8240.11489685079444757No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG8190.1141996611658405No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG7870.10973764754275514No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA7610.10611226147399831No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA7560.10541507184539123No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT7380.10290518918240574No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA7370.10276575125668432No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT7180.10011643066797737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC3100.0114.639553
GCAGGTA3150.0112.819882
AGTAGAA5700.0111.9794161
AGCGAAA1250.098.643691
GGTACTG3800.091.613135
AGCGAGC651.6370905E-1189.239174145
AGGTACT4700.078.6995854
CGAAAGC1700.072.527073
GTAGAAA9000.070.915352
GCGAAAG1900.064.892642
TAGATAT851.3007593E-859.7281723
GACAGTA3650.057.6001327
TTTAAAC10050.057.0113263
ATATTTA902.0423613E-856.409946
TAGAAAC12350.054.615523
TTTTAAA10800.053.05222
ATATTCA6900.052.5558434
CAAGGTA2500.052.197649
ACAATTC11500.050.4325038
TATATTC7150.049.703863
Produced by FastQC (version 0.11.5)