FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA50.2508780732563974No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT30.15052684395383845No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT30.15052684395383845No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.15052684395383845No Hit
GACTGAGCCCTGAGGCCCACGGGGAGGTGTGTGCAGCCTCGGGGAGCTGG20.10035122930255895No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20.10035122930255895No Hit
GTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAACTGGCATATCTTAC20.10035122930255895No Hit
AATCAATCTTCTGGTTGCTTTTCTGAGTATAGCTGTTGCCCTTTTTCCTA20.10035122930255895No Hit
CCCATATTTCAACTCTTAAGCGGGAAGAAAATGTTGACACCTGCTGTACC20.10035122930255895No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC20.10035122930255895No Hit
GTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGC20.10035122930255895No Hit
ATCCTAGAGGAAGCCTTTCAAAATACACATCACACAATGTCACTTGCTCA20.10035122930255895No Hit
AATGTAAGCTTTCCGTAGAGTGTCTGAGCCTCTGACAAGAGTTGTAAAAC20.10035122930255895No Hit
GAAATACTTTGTGATTCTACTTAACACAAGGTGCCTAGAGTCCTCAAGCT20.10035122930255895No Hit
AAAATGACCATCGTCAGCATCCACAGCATTCTGCTGTTCCTCTCGATATT20.10035122930255895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAAT100.006560914145.06
Produced by FastQC (version 0.11.5)