FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011523
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG12730.12584983238146835No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT11780.11645805384553787No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA11290.11161387333753162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.057.3464431
TATACGT1251.26238065E-534.810944
CGAAAGC3950.031.2123432
CAGCTCG6300.031.0704429
GCGAAAG4250.029.0105531
TATACAG9650.027.8066675
GTATATA15700.024.945381
TATATAC17950.024.645633
ATATACT12550.023.6927074
TATACTT13300.022.9019365
GATCCGT2903.2761818E-722.5070746
CTTACAC10800.022.1520779
TACTTAC11650.021.1581277
ATACGTG2104.3050363E-420.7207985
CAATTCG1758.4926614E-720.7136299
TACAGCT10400.020.2157067
GTACACC3951.4068064E-820.1962223
TATACTG6850.020.1158125
TGTATAC3651.3556928E-719.869262
TAGGGTG3001.1025482E-519.3394135
Produced by FastQC (version 0.11.5)