FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCAACAAAATAAAATCAGAGAAGACACACATTCACATCTTTTCCTTCA13.225806451612903No Hit
CTGTGCCGCCATGTATGAATTTCAGATGATACCAATGATAAGCAAATGTA13.225806451612903No Hit
GGCCAGCTTAAATTTTAATTCTTCGTATCTATTGTGAGTCCGGGTGAAAC13.225806451612903No Hit
GTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATTTTAAA13.225806451612903No Hit
ACATGAACCCAAGCACTGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAA13.225806451612903No Hit
TCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGA13.225806451612903No Hit
AGATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCAAACTGAG13.225806451612903No Hit
CAATTGAAGAAAAATTTGATATTACAGCAACTATGCTCAAGCATGCAGAC13.225806451612903No Hit
GTTCTCCACAGTATTTGAGTGTCTCCCCGAAGCCACCTAATACAATATGT13.225806451612903No Hit
GGTTTGCCCTATTGACAAAGTTCAGATCGCCCCTAACTGCCTTGATCATG13.225806451612903No Hit
AATAGATTGCATCAATTATGGTACAAATGTTAAACATCAAGACTAACAAT13.225806451612903No Hit
CTTAAAGAATCTAAATGAATCAATGCAAAACGAGGAAATAGAGAAAACAA13.225806451612903No Hit
GGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCC13.225806451612903No Hit
TCTCCTATTTAAAGAACACAGTATTTTTCACATTTGTTTGGCTAAAGTCT13.225806451612903No Hit
AAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTC13.225806451612903No Hit
TGATCATCCTGATCAACTCCAACACCATTGTTCCAACTCCTTTGACTGCA13.225806451612903No Hit
TACCCACTGGCTACGGCAGGTCCATACACACATGCAGGCAGGCAGGACAT13.225806451612903No Hit
AAAAATATCATGAATTAAGAATCAAGCGAGAAACAAACAATGAAAAAAGC13.225806451612903No Hit
ATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTT13.225806451612903No Hit
TTAGATAACTTCTTTTGTTTAATCTATGGTTCTTTAACACTATTGTTCTT13.225806451612903No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG13.225806451612903No Hit
CAACTTGATATTAAAAACACTATAGACCAACGCCCCGAAGGGGACGAAAA13.225806451612903No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACAAGCGAAAGGGTACAACTT13.225806451612903No Hit
GCGCTAGGATGAGTCCCAAGAGTTCTCAGTGAATGTCCCAACTGCCTAGT13.225806451612903No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA13.225806451612903No Hit
ACTATGCACAAATTTGGAAGTTTGTTTCATGTATTCGGATTTCCCTTTCA13.225806451612903No Hit
CTCCAGAACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA13.225806451612903No Hit
GTCAGCACTCATTCTGAGGGGATCAGTTGCACATAAATCATCCCTGCCTG13.225806451612903No Hit
GGCCAGAACCATTCTGTTCTCATGTCTTATTAATGGATTGGTTGTTGTCA13.225806451612903No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13.225806451612903No Hit
TCCATAATCACATCCTGTGCCTCCTAGGCTTCGAGATCAGCATGTCCAGG13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)